文献类型：期刊文献

英文题名：LcERF19, an AP2/ERF transcription factor from Litsea cubeba, positively regulates geranial and neral biosynthesis

作者：Wang, Minyan[1,2] Gao, Ming[1,2] Zhao, Yunxiao[1,2] Chen, Yicun[1,2] Wu, Liwen[1,2] Yin, Hengfu[1,2] Xiong, Shifa[1,2] Wang, Siqi[1,2] Wang, Jue[1,2] Yang, Yang[1,2] Wang, Jia[1,2] Wang, Yangdong[1,2]

通信作者：Wang, YD[1];Wang, YD[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China

年份：2022

卷号：9

外文期刊名：HORTICULTURE RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000837070400002)】；

基金：Thisworkwas supported by the National Natural Science Foundation of China (31370576).

语种：英文

摘要：The APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factors (TFs) are involved in the regulation of specialized terpenoid biosynthesis. However, the AP2/ERF TFs in Litsea cubeba have not been characterized and their role in the biosynthesis of terpenoids is unknown. Here, 174 LcAP2/ERF TFs were identified in L. cubeba and categorized into four subfamilies: 27 AP2, 7 RAV, 1 Soloist, and 139 ERF. Transcriptomic and qRT-PCR assays both showed that the expression levels of LcERF19 were similar to that of terpene synthase LcTPS42 in the pericarp, which is related to the synthesis of geranial and neral in L. cubeba. LcERF19 was further shown to encode a nuclear-localized protein and its expression was strongly induced by jasmonate. Yeast one-hybrid and dual-luciferase assays showed that LcERF19 associated with GCC box elements of the LcTPS42 promoter and promoted its activity. Transient overexpression of LcERF19 in L. cubeba and overexpression of LcERF19 in tomato resulted in a significant increase in geranial and neral. Our findings show that LcERF19 enhances geranial and neral biosynthesis through activation of LcTPS42 expression, which provides a strategy to improve the flavor of tomato and other fruits.