文献类型：期刊文献

英文题名：Seed endophytic microbiota in a coastal plant and phytobeneficial properties of the fungus Cladosporium cladosporioides

作者：Qin, Yuan[1] Pan, Xueyu[1] Yuan, Zhilin[1]

第一作者：Qin, Yuan

通信作者：Yuan, ZL[1]

机构：[1]Chinese Acad Forestry, Inst Subtrop Forestry, Hangzhou, Zhejiang, Peoples R China

年份：2016

卷号：24

起止页码：53-60

外文期刊名：FUNGAL ECOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000412379400008)】；

基金：This research was supported financially by the National Natural Science Foundation of China (No. 31370704) and the Non-Profit Sector Special Research Fund of the Chinese Academy of Forestry (RISF2013005). We would like to extend our sincerest thanks and great appreciation to Dr. Ryoko Oono, the University of California (Santa Barbara) for her valuable comments.

语种：英文

外文关键词：Seed-borne endophytes; Epiphytic fungi; Vertical transmission; Spermosphere

摘要：While the structure and function of the microbial communities associated with the rhizosphere and phyllosphere have received broad attention, the knowledge of seed-associated microbiota still remains limited. In this work, we characterized the seed fungal endophytes present in a coastal plant Suaeda salsa using 454 pyrosequencing. The fungal community present in its seeds exhibited extremely low species richness. Very few genera were identified, and the genus Cladosporium dominated. In parallel, we recovered one isolate of Cladosporium cladosporioides from the seeds. Hence, we hypothesized that the endophytic C. cladosporioides would exert a beneficial effect on plant fitness. Subsequent inoculation test revealed the role of C. cladosporioides in improving host seed germination rates. Re-isolation assay and scanning electronic microscopy analysis of its colonization pattern during seed germination, coupled with our previous observation that C cladosporioides was also present in the phyllosphere, rhizosphere and root endosphere of S. salsa, supported the evidence of its primary soil-borne origin and both epiphytic and endophytic infection of host tissues. Furthermore, significant growth enhancement was recorded in American sweetgum seedlings inoculated with an optimal conidia concentration of C. cladosporioides. In conclusion, our work undersoires the ecological significance of seed -borne endophytes and the system presented can be developed into a tool for understanding endophytic associations in the costal environments. (C) 2016 Elsevier Ltd and British Mycological Society. All rights reserved.