文献类型：期刊文献

英文题名：ThNAC12 from Tamarix hispida directly regulates ThPIP2;5 to enhance salt tolerance by modulating reactive oxygen species

作者：Wang, Rui[1,2] Zhang, Yu[1] Wang, Chao[2] Wang, Yu-Cheng[2] Wang, Liu-Qiang[1,3]

第一作者：Wang, Rui

通信作者：Wang, LQ[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,State Forestry A, Beijing 100091, Peoples R China;[2]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China;[3]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Peoples R China

年份：2021

卷号：163

起止页码：27-35

外文期刊名：PLANT PHYSIOLOGY AND BIOCHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000648298300004)】；

基金：This work was supported by the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (No. CAFYBB2018QB002) and National Natural Science Foundation of China (No. 31600539 and 31971620).

语种：英文

外文关键词：Antioxidant enzyme; NACRS; NAC transcription factor; ROS; Salt stress; Tamarix; ThNAC12

摘要：NAC (NAM, ATAF1/2 and CUC2) transcription factors play critical roles in plant development and abiotic stress responses, and aquaporins have diverse functions in environmental stress responses. In this study, we described the salt-induced transcriptional responses of ThNAC12 and ThPIP2;5 in Tamarix hispida, and their regulatory mechanisms in response to salt stress. Using yeast one-hybrid (Y1H), chromatin immunoprecipitation (ChIP) and dual-luciferase reporter assays, we identified that ThNAC12 directly binds to the NAC recognition sequence (NACRS) of the ThPIP2;5 promoter and then activates the ThPIP2;5 expression. Subcellular localization and transcriptional activation assays demonstrated that ThNAC12 was a nuclear protein with a C-terminal transactivation domain. Compared with the corresponding control plants, transgenic plants overexpressing ThNAC12 exhibited enhanced salt tolerance and displayed increased reactive oxygen species (ROS) scavenging capability and antioxidant enzyme activity levels under salt stress. All results suggested that overexpression of ThNAC12 in plants enhanced salt tolerance through modulation of ROS scavenging via direct regulation of ThPIP2;5 expression in T. hispida.