文献类型：期刊文献

中文题名：木麻黄NHX基因家族的鉴定及盐胁迫下的表达分析

英文题名：Identification of NHX Gene Family and Their Responses to Salt Stress in Casuarina equisetifolia

作者：艾迪[1,2] 魏永成[1] 孟景祥[1] 张捷[2] 仲崇禄[1] 张勇[1]

第一作者：艾迪

机构：[1]中国林业科学研究院热带林业研究所,国家林草局重点实验室,广州510520;[2]东北林业大学园林学院,哈尔滨150040

年份：2023

卷号：43

期号：5

起止页码：750-760

中文期刊名：西北植物学报

外文期刊名：Acta Botanica Boreali-Occidentalia Sinica

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：福建省林业种苗科技攻关七期项目(ZMGG-0704)。

语种：中文

中文关键词：木麻黄;NHX基因家族;生物信息学分析;盐胁迫

外文关键词：Casuarina equisetifolia;NHX gene family;bioinformation analysis;salt stress

分类号：Q786;Q789;S792.93

摘要：Na^(+)/H^(+)转运蛋白在植物的耐盐性和生长发育中起关键作用。该研究采用生物信息学方法对木麻黄(Casuarina equisetifolia L.)NHX基因家族成员进行全基因组鉴定和分析,并利用qRT-PCR技术检测NHX基因在盐胁迫下的表达,以探讨木麻黄NHX家族基因的生物学功能,为进一步研究盐胁迫机制以及挖掘其抗逆基因奠定理论基础。结果显示:(1)鉴定获得8个木麻黄NHX基因家族成员(CeqNHX1-8),系统进化树分析发现其可分为Endo、Vac和PM共3个亚家族,分别包含1、5和2个基因;编码氨基酸数为324~546个,分子量为34.87~60.27 kD,等电点在6.29~9.08之间,均为疏水蛋白。(2)基因结构和Motif分析发现,所有CeqNHXs基因的外显子数为2~17不等,且均具有保守的Na^(+)/H^(+)交换结构域;CeqNHX基因的蛋白质二级结构主要以α-螺旋和不规则卷曲为主。(3)木麻黄CeqNHX基因的启动子区含有大量非生物胁迫和激素响应元件,其中CeqNHX5启动子共含有13种共38个元件。(4)qRT-PCR分析表明,CeqNHX基因家族成员在木麻黄根、茎、枝和雄花序中均有不同程度的表达,且多数主要在小枝上表达;在200 mmol/L NaCl处理下,8个木麻黄CeqNHXs基因的表达量均有一定程度的上调。研究表明,木麻黄CeqNHXs基因家族可能参与多种激素和应激反应的调节,且CeqNHXs基因的表达对盐胁迫有显著的响应,推测CeqNHXs基因与木麻黄的耐盐性相关。
Salinity is one of the major environmental factors that limit the growth and development of plants worldwide.It has been documented that Na^(+)/H^(+)transporters play a crucial role in salt tolerance and the development of plants.In this study,the genome-wide of NHX genes of Casuarina equisetifolia L.were identified and analyzed by bioinformatics method,and the expression of NHX genes in C.equisetifolia under salt stress was detected by qRT-PCR for exploring the biological function of CeqNHX gene family,which laid a theoretical foundation for further research on salt stress mechanism and digging out its resistance genes.The results were summarized as follows:(1)eight CeqNHXs(CeqNHX1-8)were identified,and phylogenetic analyses showed that they belong to three subfamilies(Endo,Vac,and PM),containing 1,5,and 2 genes,respectively;the number of coding amino acids were 324-546,and the molecular weight were 34.87-60.27 kD,the theoretical isoelectric points were between 6.29-9.08,all of which were hydrophobic amino acids.(2)Gene structure and motif analysis revealed that the number of exons range from 2 to 17,and all contain Na^(+)/H^(+)exchange conserved domains;the secondary structure were mainly composed ofα-helix and random coil.(3)The promoter regions of CeqNHX genes contain a large number of abiotic stress and hormone response elements,among which,the CeqNHX5 promoter contains 13 kinds and 38 elements.(4)The results of qRT-PCR showed that the CeqNHX genes were expressed differently in different tissues,and most members were mainly expressed in shoots.Under NaCl stress condition,the expression of 8 CeqNHXs in C.equisetifolia was up-regulated to a certain extent.Studies had shown that CeqNHX genes might be involved in the regulation of various hormones and stress responses,and the expression of CeqNHXs was significantly responsive to salt stress,suggesting that CeqNHXs might be related to salt tolerance of C.equisetifolia.