文献类型：期刊文献

中文题名：无距虾脊兰花粉离体萌发及储藏条件的研究

英文题名：in vitro Pollen Germination and Storage Conditions of Calanthe tsoongiana

作者：钱鑫[1] 刘芬[2] 牛晓玲[3] 王彩霞[1] 田敏[1]

第一作者：钱鑫

机构：[1]中国林业科学研究院亚热带林业研究所;[2]中国科学院华南植物园;[3]天目山国家自然保护区管理局

年份：2014

卷号：34

期号：2

起止页码：341-348

中文期刊名：西北植物学报

外文期刊名：Acta Botanica Boreali-Occidentalia Sinica

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：浙江省花卉育种重大科技项目(2012C12909-10)

语种：中文

中文关键词：无距虾脊兰;花粉;生活力;储藏性

外文关键词：Calanthe tsoongiana ; pollen; pollen viability ; storage capacity

分类号：Q813.11;Q944.42

摘要：以天目山野生无距虾脊兰的花粉为材料,采用离体萌发法研究了花粉的储藏性以及不同的培养基组分和培养条件对花粉萌发和花粉管生长的影响,并采用联苯胺-过氧化氢法测定了其柱头的可授性,以筛选无距虾脊兰花粉的培养方法以及储藏条件,为其种质资源保存等研究奠定基础.结果表明：（1）无距虾脊兰最适花粉液体培养基为200 g·L-1蔗糖＋50 mg·L-1 H3BO3＋40 mg·L-1 Ca（NO3）2·4H2O,并且在pH 5.5～6.0、温度25℃恒温下培养48 h,无距虾脊兰花粉萌发率（81.71％）和花粉管生长（247.42 μm）最佳.（2）无距虾脊兰花粉在-80℃中低温干燥储藏360 d后仍具有48.58％的萌发率.（3）无距虾脊兰的柱头在开花前5d内均具有可授性,花粉块在整个花期内均保持了28.96％～81.71％的生活力,但柱头可授性和花粉活力均随开花后时间的延长显著降低.
The effects of medium components and culture conditions on pollen germination and pollen tube growth of Calanthe tsoongiana T. Tang et F. T. Wang in Tianmu Mountain were studied by in vitro culture ,which was also used to investigate the pollen storage capacity. The stigma receptivity was estimated by benzidine-H2O2 method. The aim of present study was to screen the ideal culture methods and storage conditions for C. tsoongiana pollens,which would lay the foundation for the research of its germplasm re- sources preservation. The results showed that： （1）The optimum culture medium for pollen germination consisted of 200 g ~ L-1 sucrose,50 mg ~ L 1 H3BOa and 40 mg ~ L-1 Ca（NOa）2 ~ 4H20,in which the pol- len germination percentage was nearly 81.71% and the average pollen tube length reached 247.42/~m after 48 h incubation under the optimum culture conditions involved pH 5.5--6.0 and 25 ℃. （2）Germination rate of dried pollens still maintained at 48.58℃ after 360 d storage under --80 ℃. （3）The stigma receptivi- ty of C. tsoongiana could last 5 d after blooming,but the pollinarium kept a certain viability （28.96%-81.71% germination rate） during whole florescence. However, both stigma receptivity and pollen viability declined significantly with the extension of time after blooming.