文献类型：期刊文献

英文题名：Populus euphratica annexin1 facilitates cadmium enrichment in transgenic Arabidopsis

作者：Zhang, Yinan[1,2] Sa, Gang[1] Zhang, Ying[1] Hou, Siyuan[1] Wu, Xia[1] Zhao, Nan[1] Zhang, Yuhong[1,3] Deng, Shurong[1,3] Deng, Chen[1] Deng, Jiayin[1] Zhang, Huilong[1] Yao, Jun[1] Zhang, Yanli[1] Zhao, Rui[1] Chen, Shaoliang[1]

第一作者：Zhang, Yinan

通信作者：Chen, SL[1]

机构：[1]Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Box 162, Beijing 100083, Peoples R China;[2]Chinese Acad Forestry, Forestry Inst New Technol, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, Key Lab Silviculture State Forestry Adm, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2021

卷号：405

外文期刊名：JOURNAL OF HAZARDOUS MATERIALS

收录：;EI(收录号：20204309403903);Scopus(收录号：2-s2.0-85093701032);WOS:【SCI-EXPANDED(收录号:WOS:000616179100005)】；

基金：The research was supported jointly by the National Natural Science Foundation of China (grant nos. 31770643, 31570587, 32071730), Beijing Natural Science Foundation, China (grant nos. 6182030, 6172024), Fundamental Research Funds for the Central Universities, China (grant no. 2019ZY25), the Central Public-Interest Scientific Institution Basal Research Fund, China (Chinese Academy of Forestry, CAFYBB2019SZ010), the Program of Introducing Talents of Discipline to Universities, China (111 Project, grant no. B13007), and Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, China (Beijing Forestry University).

语种：英文

外文关键词：Annexins; Arabidopsis thaliana; Calcium-permeable channels; Cd2+ flux; Populus euphratica

摘要：Phytoremediation offers a great potential for affordable remediation of heavy metal (HM)-polluted soil and water. Screening and identifying candidate genes related to HM uptake and transport is prerequisite for improvement of phytoremediation by genetic engineering. Using the cadmium (Cd)-hypersensitive Populus euphratica, an annexin encoding gene facilitating Cd enrichment was identified in this study. With a 12 h exposure to CdCl2 (50-100 mu M), P. euphratica cells down-regulated transcripts of annexin1 (PeANN1). PeANN1 was homologue to Arabidopsis annexin1 (AtANN1) and localized mainly to the plasma membrane (PM) and cytosol. Compared with wild type and Atann1 mutant, PeANN1 overexpression in Arabidopsis resulted in a more pronounced decline in survival rate and root length after a long-term Cd stress (10 d, 50 mu M), due to a higher cadmium accumulation in roots. PeANN1-transgenic roots exhibited enhanced influx conductance of Cd2+ under cadmium shock (30 min, 50 mu M) and short-term stress (12 h, 50 mu M). Noteworthy, the PeANN1-facilitated Cd2+ influx was significantly inhibited by a calcium-permeable channel (CaPC) inhibitor (GdCl3) but was promoted by 1 mM H2O2, indicating that Cd2+ entered root cells via radical-activated CaPCs in the PM. Therefore, PeANN1 can serve as a candidate gene for improvement of phytoremediation by genetic engineering.