文献类型：期刊文献

中文题名：单叶省藤萌蘖芽组培快繁技术及试管内开花

英文题名：Rapid Propagation and Flowering of Calamus simplicifolius in Vitro

作者：李湘阳[1] 曾炳山[1] 裘珍飞[1] 刘英[1]

第一作者：李湘阳

机构：[1]中国林科院热带林业研究所

年份：2007

卷号：32

期号：2

起止页码：1-3

中文期刊名：林业科技

外文期刊名：Forestry Science & Technology

收录：北大核心:【北大核心2004】；

基金：国家高技术研究发展计划(863计划)项目(2006AA100109);"十一五"国家科技支撑项目"棕榈藤无性快繁工艺研究"

语种：中文

中文关键词：单叶省藤;萌蘖芽;外植体;组培快繁;试管开花

外文关键词：Calamus simplicifolius ; Sucker; Explant ; Tissue culture; Tube flowering

分类号：S792.91;S722.37

摘要：以单叶省藤萌蘖芽为外植体进行组织培养,在完善外植体采集技术、消毒技术及防褐变技术基础上,用改良MS+6-BA 8mg/L+IBA 1mg/L+2,4-D 0.75mg/L+抗坏血酸10mg/L+半胱氨酸2mg/L培养基诱导芽分化,MS+NAA1mg/L+IBA 2mg/L培养基诱导根分化,改良MS+6-BA 4mg/L+IBA 0.5mg/L+2,4-D0.4mg/L+活性炭0.2g/L培养基诱导花芽分化,实现了单叶省藤萌蘖芽的组培快繁,并在试管内开花。本研究不仅为单叶省藤的良种快繁奠定了基础,也为深入研究外界条件对单叶省藤性别分化的调控提供了一个简便的实验体系。
This study deals with the tissue culture method of sucker explant from Calamus simplicifolius. The technique of collection, sterilization and anti - browning of sucker explant were perfected. Modified MS medium with 6 - BA 8mg/L ＋ IBA lmg/L ＋ 2, 4 - D 0. 75mg/L ＋ vitamin C 10mg/L ＋ cysteine 2mg/L induced multiple shoots. Roots were formed on modified MS medium containing NAAlmg/L ＋ IBA 2mg/L. And modified MS medium with 6 - BA 4mg/L ＋ IBA0. 5mg/L ＋ 2, 4 - D 0. 4mg/L ＋ active carbon 0. 2g/L induced flowering. This research was very useful for clonal selection and propagation. The result also gave a clue for application of flowering of Calamus simplicifolius, in vitro and developed system for studying physiological mechanism of sex differentiation.