文献类型：期刊文献

英文题名：Effects of long-term increased N deposition on tropical montane forest soil N-2 and N2O emissions

作者：Tang, Wenguang[1,2] Chen, Dexiang[1] Phillips, Oliver L.[3] Liu, Xian[4] Zhou, Zhang[1] Li, Yide[1] Xi, Dan[4] Zhu, Feifei[2,7] Fang, Jingyun[5] Zhang, Limei[6] Lin, Mingxian[1] Wu, Jianhui[1] Fang, Yunting[2,7]

第一作者：Tang, Wenguang

通信作者：Chen, DX[1];Fang, YT[2]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Jianfengling Natl Key Field Observat & Res Stn Fo, Guangzhou 510520, Guangdong, Peoples R China;[2]Chinese Acad Sci, Inst Appl Ecol, CAS Key Lab Forest Ecol & Management, Shenyang 110016, Liaoning, Peoples R China;[3]Univ Leeds, Sch Geog, Leeds LS2 9JT, W Yorkshire, England;[4]Fujian Agr & Forestry Univ, Coll Forestry, Fuzhou 350002, Fujian, Peoples R China;[5]Peking Univ, Dept Ecol, Coll Urban & Environm Sci, Key Lab Earth Surface Proc,Minist Educ, Beijing 100871, Peoples R China;[6]Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China;[7]Chinese Acad Sci, Qingyuan Forest CERN, Shenyang 110014, Liaoning, Peoples R China

年份：2018

卷号：126

起止页码：194-203

外文期刊名：SOIL BIOLOGY & BIOCHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000447580800022)】；

基金：This work was financially supported by the National Key Research and Development Program of China (No. 2016YFA0600800), Fundamental Research Funds for the Central Non-profit Research Institution of CAF (Nos. CAFYBB2016ZD002, RITFYWZX201401, RITFYWZX201404), the Strategic Priority Research Program of Chinese Academy of Sciences (No. XDB15020200), the National Natural Science Foundation of China (Nos. 31422009, 41773094, 31400422 and 31370464), and the Key Research Program of Frontier Sciences of Chinese Academic of Sciences (No. QYZDB-SSW-DQC002). OLP acknowledges the support of the ERC Advanced Grant 291585 (T-FORCES) and a Royal Society-Wolfson Research Merit Award. The work was also jointly supported by the Jianfengling National Key Field Station. We thank Sarah A. Batterman at University of Leeds and James A. Hogan at Florida International University for their constructive comments on an earlier version of the manuscript. We also thank Ruming Peng and other colleagues in Jianfengling National Field Station for their assistance in field work, and Xiaoming Fang, Ang Wang, Lu Cheng, Xianfeng Wang, Ying Tu, Dongwei Liu, Linlin Song, and Zhengjie Li for their skillful assistance in the laboratory. The authors declare no conflict of interests.

语种：英文

外文关键词：Nitrogen deposition; Tropical montane forests; Nitrous oxide emission; Dinitrogen emission; Denitrification; Denitrification genes

摘要：Nitrogen (N) deposition is projected to substantially increase in the tropics over the coming decades, which is expected to lead to enhanced N saturation and gaseous N emissions from tropical forests (via NO, N2O, and N-2). However, it is unclear how N deposition in tropical forests influences both the magnitude of gaseous loss of nitrogen and its partitioning into the N-2 and N2O loss mechanisms. Here, for the first time, we employed the acetylene inhibition technique and the N-15-nitrate labeling method to quantify N-2 and N2O emission rates for long-term experimentally N-enriched treatments in primary and secondary tropical montane forest. We found that during laboratory incubation under aerobic conditions long-term increased N addition of up to 100 kg N ha(-1) yr(-1) at Jianfengling forest, China, did not cause a significant increase in either N2O or N-2 emissions, or N2O/N-2. However, under anaerobic conditions, N2O emissions decreased and N-2 emissions increased with increasing N addition in the secondary forest. These changes may be attributed to substantially greater N2O reduction to N-2 during denitrification, further supported by the decreased N2O/N-2 ratio with increasing N addition. No such effects were observed in the primary forest. In both forests, N addition decreased the contribution of denitrification while increasing the contribution of co-denitrification and heterotrophic nitrification to N2O production. Denitrification was the predominant pathway to N-2 production (98-100%) and its contribution was unaffected by N addition. Despite the changes in the contributions of denitrification to N2O gas emissions, we detected no change in the abundance of genes associated with denitrification. While the mechanisms for these different responses are not yet clear, our results indicate that the effects of N deposition on gaseous N loss were ecosystem-specific in tropical forests and that the microbial processes responsible for the production of N gases are sensitive to N inputs.