文献类型：期刊文献

英文题名：LkCNR6, a member of Cell number regulator genes from Larix kaempferi, confers zinc stress tolerance in transgenic Arabidopsis

作者：Zhang, Xu[1] Li, Yu[1] Yun, Chengrong[1] Sun, Wenting[1] Fan, Zhoupeng[1] Xie, Yunhui[2] Wang, Chunguo[1]

第一作者：Zhang, Xu

通信作者：Wang, CG[1];Xie, YH[2]

机构：[1]Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry &, Res Inst Forestry, Beijing 100091, Peoples R China

年份：2025

卷号：161

期号：1

外文期刊名：PLANT CELL TISSUE AND ORGAN CULTURE

收录：;EI(收录号：20251418166841);Scopus(收录号：2-s2.0-105001483735);WOS:【SCI-EXPANDED(收录号:WOS:001457331100003)】；

基金：This work was supported by the grants from the Sci-Tech Innovation 2030 Agenda (No. 2023ZD0405903), the Science and Technology Foundation of Tianjin, China (No. 23YFZCSN00190 and 23ZXZYSN00080), the National Key Research and Development Program of China (No. 2022YFD2200302) and the Natural Science Foundation of China (No. 31872115).

语种：英文

外文关键词：Cell number regulator (CNR); Larix kaempferi; Heavy metal stress; Zinc transport; Organ development

摘要：Larix kaempferi, one of the representative gymnosperms, is the main afforestation and timber species in the world. As one essential micronutrient, zinc plays a vital role in Larix kaempferi growth, yet its overaccumulation causes substantial growth inhibition. However, the underlying mechanism of zinc stress response in Larix kaempferi is still not well elucidated. In the present study, a potential zinc stress-related gene, LkCNR6 (Cell number regulator 6) was identified in Larix kaempferi. LkCNR6 was confirmed to localize in the plasma membrane and displayed rapidly inducible expression patterns under zinc stress. To uncover the roles of LkCNR6, the LkCNR6-overexpressing transgenic Arabidopsis lines were created. The phenotypic data confirmed that ectopic overexpression of LkCNR6 significantly improved zinc stress tolerance in Arabidopsis, although the LkCNR6-overexpressing transgenic plants displayed slower growth rate and smaller aerial organ size than that of the wild-type plants. Zinc accumulation assay indicated that the improved tolerance to zinc stress was mostly attributed to increase zinc excretion in the overexpressing LkCNR6 transgenic plants. Furthermore, yeast two-hybrid assay identified that LkHMP1, LkWAKL4 and LkRTFL, were physically interacted with LkCNR6. The interaction between LkCNR6 and LkHMP1, a protein associated with metal transport, was further confirmed by bimolecular fluorescence complementation (BiFC) assay. In summary, these results demonstrated that LkCNR6 plays important roles in positively responding zinc stress and negatively regulating organ development. These findings provide new insights into the regulatory mechanism of LkCNR6-mediated zinc stress response, and suggest the application of LkCNR6 in breeding high-heavy metal resistance crops.