文献类型：期刊文献

英文题名：A transcriptome-based association study of growth, wood quality, and oleoresin traits in a slash pine breeding population

作者：Ding, Xianyin[1] Diao, Shu[1,2] Luan, Qifu[1,2] Wu, Harry X.[2,3,4] Zhang, Yini[1] Jiang, Jingmin[1,2]

第一作者：Ding, Xianyin

通信作者：Luan, QF[1];Luan, QF[2]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou, Peoples R China;[2]Natl Forestry & Grassland Engn Technol Res Ctr Ex, Hangzhou, Peoples R China;[3]Swedish Univ Agr Sci, Umea Plant Sci Ctr, Dept Forest Genet & Plant Physiol, Umea, Sweden;[4]CSIRO Natl Collect Res Australia, Black Mt Lab, Canberra, ACT, Australia

年份：2022

卷号：18

期号：2

外文期刊名：PLOS GENETICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000871831900001)】；

基金：QL is supported by a Fundamental Research Funds of Chinese Forestry Academy (CAFYBB2017ZA001-2-1) and a Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding (2021C02070-8). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

摘要：Slash pine (Pinus elliottii Engelm.) is an important timber and resin species in the United States, China, Brazil and other countries. Understanding the genetic basis of these traits will accelerate its breeding progress. We carried out a genome-wide association study (GWAS), transcriptome-wide association study (TWAS) and weighted gene co-expression network analysis (WGCNA) for growth, wood quality, and oleoresin traits using 240 unrelated individuals from a Chinese slash pine breeding population. We developed high quality 53,229 single nucleotide polymorphisms (SNPs). Our analysis reveals three main results: (1) the Chinese breeding population can be divided into three genetic groups with a mean inbreeding coefficient of 0.137; (2) 32 SNPs significantly were associated with growth and oleoresin traits, accounting for the phenotypic variance ranging from 12.3% to 21.8% and from 10.6% to 16.7%, respectively; and (3) six genes encoding PeTLP, PeAP2/ERF, PePUP9, PeSLP, PeHSP, and PeOCT1 proteins were identified and validated by quantitative real time polymerase chain reaction for their association with growth and oleoresin traits. These results could be useful for tree breeding and functional studies in advanced slash pine breeding program.