文献类型：期刊文献

中文题名：基于RAD高通量测序的蓝花楹群体遗传分析

英文题名：Population Genetic Analysis of Jacaranda mimosifolia by RAD Hight Throughput Sequencing Technique

作者：刘学锋[1] 李小梅[2] 张国武[1] 张沛健[1] 刘果[1] 黄敏[1] 陈银霞[3]

第一作者：刘学锋

机构：[1]中国林业科学研究院速生树木研究所,广东湛江524022;[2]湛江科技学院,广东湛江524000;[3]江西省林业科技推广和宣传教育中心,南昌330033

年份：2022

卷号：30

期号：5

起止页码：613-622

中文期刊名：热带亚热带植物学报

外文期刊名：Journal of Tropical and Subtropical Botany

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD_E2021_2022】；

基金：中央级公益科研院所专项资金项目(CAFYBB2019MB004);广东省林业科技创新项目(2018KJCX024)资助。

语种：中文

中文关键词：蓝花楹;RAD-seq;遗传多样性;群体遗传

外文关键词：Jacaranda mimosifolia;RAD-seq;Genetic diversity;Population genetic

分类号：S685.99

摘要：为了解蓝花楹(Jacaranda mimosifolia)种质资源的遗传多样性和群体遗传结构,对168份种质材料进行RAD-seq测序,构建了系统进化树并进行主成分、群体结构和遗传多样性分析。结果表明,比对参考基因组平均比对率为81.02%,平均测序深度23.18×,最终获得45552个高质量的SNPs。群体遗传结构分析表明,供试蓝花楹可划分为2个大的类群,来自川、渝地区的种质材料基本归为一类;其余地区归为另一类。19个地区的蓝花楹在SNP水平上的遗传多样性较高,云南昆明(YNKM)居群的核苷酸多样性(π)和期望杂合度(He)最大,表现出最高的遗传多样性。因此,来自川、渝地区的蓝花楹具有相对较近的亲缘关系,推断来自同一祖先,而其余地区的种质可能是随机引种栽培。
In order to understand the genetic diversity of Jacaranda mimosifolia germplasm resources,168 germplasms of Jacaranda mimosifolia were restriction-site associated DNA sequencing(RAD-seq),and then the phylogenetic tree was constructed,principal component(PCA),population structure and genetic diversity were analyzed.The results showed that the mean alignment rates to the reference genomes were 81.02%with an average sequencing depth of 23.18×.After cleaned and filtered,a total of 45552 SNPs were obtained.All tested germplasms could be clustered into two groups,those from Sichuan-Chongqing region were in one group,and the rest were in another group.Jacaranda mimosifolia has a high genetic diversity at the SNP level.Among them,nucleotide diversity(π)and expected heterozygosity(He)in YNKM population is the largest,showing the highest genetic diversity.Therefore,Jacaranda mimosifolia from Sichuan-Chongqing region showed relatively close genetic relationship,suggesting that they might from the same ancestor,and those from other regions might be randomly introduced and cultivated.