文献类型：期刊文献

英文题名：Metabolome and Transcriptome Analyses Uncover the Molecular Mechanism of Massive Accumulation of Secondary Metabolites in Fenugreek (Trigonella Foenum-Gracum) Seed

作者：Zhao, Qiuyu[1] Wu, Guo-Xing[2] Shi, Yuan-Chong[3,4] Fu, Zuo-Yi[3,4] Mo, Hai-Feng[3,4] Shi, Chun-Lan[2] Yu, Shu-Hui[1]

第一作者：Zhao, Qiuyu

机构：[1] College of Agriculture and Life Sciences, Kunming University, Kunming, 650214, China; [2] College of Plant Protection, Yunnan Agricultural University, Kunming, 650201, China; [3] Institute of Highland Forest Science, Chinese Academy of Forestry, Kunming, 650224, China; [4] Key Laboratory of Breeding and Utilization of Resource Insects of National Forestry and Grassland Administration, Kunming, 650224, China

年份：2023

外文期刊名：SSRN

收录：EI(收录号：20230415903)

语种：英文

外文关键词：Alkaloids - Biochemistry - Biomolecules - Biosynthesis - Flavonoids - Fungi - Gene expression - Lipids - Negative ions - Positive ions

摘要：Fenugreek (Trigonella foenum-graecum) is a traditional medicinal plant for treating human diseases and widely cultured in many countries. But there is still not clear about the ingredients and related metabolic pathways. To understand the ingredients and related gene expression changes during the seed development, this study employed metabolome and transcriptome sequencing analyses and integrative analysis to explore the metabolites and pathways during the growth of fenugreek. And the antifungal activity of fenugreek seed was also analyzed. The results showed that, there were total of 9,499 metabolites were identified in positive ion model, and 8,043 metabolites were identified in negative ion model. Among them, main ingredients of were the fatty acyls, prenol lipids, steroids and steroid derivatives, flavonoids, isoflavonoids. Among these enriched pathways, the top 20 pathways were "flavone and flavonol biosynthesis", "isoflavonoid biosynthesis", "flavonoid biosynthesis", and so on. 3,7-Di-O-methylquercetin in flavonoids, pseudobaptigenin in isoflavonoids and methyl ecgonine in alkaloids and derivatives were the most significantly up-regulated metabolites. There were 38,137 differentially expressed genes in transcriptome analysis. In KEGG pathway enrichment analysis, there were 147 DEGs were significantly enriched in "flavonoid biosynthesis". And there were 10 DEGs of 6 key enzymes were found involved in the three pathways which about flavonoids and alkaloids synthesis in fenugreek. The antifungal activity test revealed the inhibitory effect of the ethanol extract of fenugreek seed to Alternaria tenuissima (Kunze)Wiltshire and Magnaporthe oryzae. It can further prove fenugreek has research value in botanical pesticides. ? 2023, The Authors. All rights reserved.