文献类型：期刊文献

英文题名：A chromosome-level reference genome of the hazelnut, Corylus heterophylla Fisch

作者：Zhao, Tiantian[1,2] Ma, Wenxu[1,2] Yang, Zhen[1,2] Liang, Lisong[1,2] Chen, Xin[2,3] Wang, Guixi[1,2] Ma, Qinghua[1,2] Wang, Lujun[2,4]

第一作者：Zhao, Tiantian;赵天田

通信作者：Ma, QH[1];Wang, LJ[2]

机构：[1]Chinese Acad Forestry, Key Lab Tree Breeding & Cultivat State Forestry &, Res Inst Forestry, 1 Dongxiaofu,Xiangshan Rd, Beijing 100091, Peoples R China;[2]Natl Hazelnut Ind Innovat Alliance, Hazelnut Engn & Tech Res Ctr State Forestry & Gra, Xiangshan Rd, Beijing 100091, Peoples R China;[3]Shandong Acad Agr Sci, Shandong Inst Pomol, 66 Longtan Rd, Tai An 271000, Shandong, Peoples R China;[4]Anhui Acad Forestry, 820 Changjiangxi Rd, Hefei 230031, Peoples R China

年份：2021

卷号：10

期号：4

外文期刊名：GIGASCIENCE

收录：;Scopus(收录号：2-s2.0-85105769018);WOS:【SCI-EXPANDED(收录号:WOS:000697758500002)】；

基金：This work was financed by the Special Investigation on Basic Resources of Science and Technology (Grant No. 2019FY100801 03) and the Special Fund for Basic Scientific Research Business of Central Public Research Institutes of the Chinese Academy of Forestry, China (Grant No. RIF-12 and CAFYBB2017ZA004-9).

语种：英文

外文关键词：Corylus heterophylla Fisch.; Genome sequences; Nanopore; Hi-C

摘要：Background: Corylus heterophylla Fisch. is a species of the Betulaceae family native to China. As an economically and ecologically important nut tree, C. heterophylla can survive in extremely low temperatures (-30 to -40 degrees C). To deepen our knowledge of the Betulaceae species and facilitate the use of C. heterophylla for breeding and its genetic improvement, we have sequenced the whole genome of C. heterophylla. Findings: Based on >64.99 Gb (similar to 175.30x) of Nanopore long reads, we assembled a 370.75-Mb C. heterophylla genome with contig N50 and scaffold N50 sizes of 2.07 and 31.33 Mb, respectively, accounting for 99.23% of the estimated genome size (373.61 Mb). Furthermore, 361.90 Mb contigs were anchored to 11 chromosomes using Hi-C link data, representing 97.61% of the assembled genome sequences. Transcriptomes representing 4 different tissues were sequenced to assist protein-coding gene prediction. A total of 27,591 protein-coding genes were identified, of which 92.02% (25,389) were functionally annotated. The phylogenetic analysis showed that C. heterophylla is close to Ostrya japonica, and they diverged from their common ancestor similar to 52.79 million years ago. Conclusions: We generated a high-quality chromosome-level genome of C. heterophylla. This genome resource will promote research on the molecular mechanisms of how the hazelnut responds to environmental stresses and serves as an important resource for genome-assisted improvement in cold and drought resistance of the Corylus genus.