文献类型：期刊文献

英文题名：Small RNA profiling reveals that an ovule-specific microRNA, cja-miR5179, targets a B-class MADS-box gene in Camellia japonica

作者：Ma, Xianjin[1,2,3] Nie, Ziyan[1,2,3] Huang, Hu[1,2,3] Yan, Chao[1,4] Li, Sijia[1,2,3] Hu, Zhikang[1,2,3] Wang, Yupeng[2,3] Yin, Hengfu[1,3]

第一作者：Ma, Xianjin

通信作者：Yin, HF[1];Yin, HF[2]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, State Key Lab Tree Genet & Breeding, Hangzhou 311400, Zhejiang, Peoples R China;[2]Nanjing Forestry Univ, Coll Informat Sci & Technol, Nanjing 210037, Jiangsu, Peoples R China;[3]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Forest Genet & Breeding, Hangzhou 311400, Zhejiang, Peoples R China;[4]Chinese Acad Forestry, Expt Ctr Subtrop Forestry, Fenyi 336600, Jiangxi, Peoples R China

年份：2023

外文期刊名：ANNALS OF BOTANY

收录：;Scopus(收录号：2-s2.0-85183472563);WOS:【SCI-EXPANDED(收录号:WOS:001090709000001)】；

基金：We would like to thank Drs Zhifeng Wang and Vincent Chiang from Northeast Forestry University, China, for providing vectors and help with experiments. We are grateful to Dr Da Luo for helpful comments.

语种：英文

外文关键词：Arabidopsis; Camellia; miR5179; floral development; MADS-box gene; BASIC PENTACYSTEINE proteins

摘要：Background and Aims The functional specialization of microRNA and its target genes is often an important factor in the establishment of spatiotemporal patterns of gene expression that are essential to plant development and growth. In different plant lineages, understanding the functional conservation and divergence of microRNAs remains to be explored.Methods To identify small regulatory RNAs underlying floral patterning, we performed a tissue-specific profiling of small RNAs in various floral organs from single and double flower varieties (flowers characterized by multiple layers of petals) in Camellia japonica. We identified cja-miR5179, which belongs to a deeply conserved microRNA family that is conserved between angiosperms and basal plants but frequently lost in eudicots. We characterized the molecular function of cja-miR5179 and its target - a B-function MADS-box gene - through gene expression analysis and transient expression assays.Key Results We showed that cja-miR5179 is exclusively expressed in ovule tissues at the early stage of floral development. We found that cja-miR5179 targets the coding sequences of a DEFICIENS-like B-class gene (CjDEF) mRNA, which is located in the K motif of the MADS-box domain; and the target sites of miR5179/MADS-box were consistent in Camellia and orchids. Furthermore, through a petal transient-expression assay, we showed that the BASIC PENTACYSTEINE proteins bind to the GA-rich motifs in the cja-miR5179 promoter region and suppresses its expression.Conclusions We propose that the regulation between miR5179 and a B-class MADS-box gene in C. japonica has a deep evolutionary origin before the separation of monocots and dicots. During floral development of C. japonica, cja-miR5179 is specifically expressed in the ovule, which may be required for the inhibition of CjDEF function. This work highlights the evolutionary conservation as well as functional divergence of small RNAs in floral development.