文献类型：期刊文献

英文题名：CRISPR/Cas9 mutated p-coumaroyl shikimate 3'-hydroxylase 3 gene in Populus tomentosa reveals lignin functioning on supporting tree upright

作者：Zhang, Sufang[1] Wang, Bo[2] Li, Qian[1] Hui, Wenkai[1] Yang, Linjie[3] Wang, Zhihua[1] Zhang, Wenjuan[1] Yue, Fengxia[3] Liu, Nian[1] Li, Huiling[1] Lu, Fachuang[3,4] Zhang, Kewei[5] Zeng, Qingyin[6] Wu, Ai-Min[1]

第一作者：Zhang, Sufang

机构：[1] Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architectures, South China Agricultural University, Guangzhou, 510642, China; [2] College of Agriculture, South China Agricultural University, Guangzhou, 510642, China; [3] State Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou, 510640, China; [4] Department of Biochemistry and Great Lakes Bioenergy Research Center, The Wisconsin Energy Institute, University of Wisconsin, Madison, WI, 53726, United States; [5] College of Chemistry and Life Sciences, Zhejiang Normal University, Zhejiang, Jinhua, 321004, China; [6] State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, 100091, China

年份：2023

卷号：253

外文期刊名：International Journal of Biological Macromolecules

收录：EI(收录号：20233814771795);Scopus(收录号：2-s2.0-85171610139)

语种：英文

外文关键词：Biochemistry - Biomolecules - Biosynthesis - Cell proliferation - Genes - Lignin - Metabolism - Metabolites - Wood

摘要：The lignin plays one of the most important roles in plant secondary metabolism. However, it is still unclear how lignin can contribute to the impressive height of wood growth. In this study, C3’H, a rate-limiting enzyme of the lignin pathway, was used as the target gene. C3’H3 was knocked out by CRISPR/Cas9 in Populus tomentosa. Compared with wild-type popular trees, c3’h3 mutants exhibited dwarf phenotypes, collapsed xylem vessels, weakened phloem thickening, decreased hydraulic conductivity and photosynthetic efficiency, and reduced auxin content, except for reduced total lignin content and significantly increased H-subunit lignin. In the c3’h3 mutant, the flavonoid biosynthesis genes CHS, CHI, F3H, DFR, ANR, and LAR were upregulated, and flavonoid metabolite accumulations were detected, indicating that decreasing the lignin biosynthesis pathway enhanced flavonoid metabolic flux. Furthermore, flavonoid metabolites, such as naringenin and hesperetin, were largely increased, while higher hesperetin content suppressed plant cell division. Thus, studying the c3’h3 mutant allows us to deduce that lignin deficiency suppresses tree growth and leads to the dwarf phenotype due to collapsed xylem and thickened phloem, limiting material exchanges and transport. ? 2023