文献类型：期刊文献

英文题名：Genome-wide analysis of day/night DNA methylation differences in &ITPopulus nigra&IT

作者：Ding, Chang-Jun[1,2] Liang, Li-Xiong[1,2] Diao, Shu[1,2] Su, Xiao-Hua[1,2] Zhang, Bing-Yu[1,2]

第一作者：丁昌俊

通信作者：Zhang, BY[1];Zhang, BY[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Key Lab Tree Breeding & Cultivat, Beijing, Peoples R China

年份：2018

卷号：13

期号：1

外文期刊名：PLOS ONE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000419101600089)】；

基金：Funding was provided by the National Nonprofit Institute Research Grant of Chinese Academy of Forestry (TGB2013010) and the "863" Project (2013AA102703).

语种：英文

摘要：DNA methylation is an important mechanism of epigenetic modification. Methylation changes during stress responses and developmental processes have been well studied; however, their role in plant adaptation to the day/night cycle is poorly understood. In this study, we detected global methylation patterns in leaves of the black poplar Populus nigra 'N46' at 8:00 and 24:00 by methylated DNA immunoprecipitation sequencing (MeDIP-seq). We found 10,027 and 10,242 genes to be methylated in the 8:00 and 24:00 samples, respectively. The methylated genes appeared to be involved in multiple biological processes, molecular functions, and cellular components, suggesting important roles for DNA methylation in poplar cells. Comparing the 8:00 and 24:00 samples, only 440 differentially methylated regions (DMRs) overlapped with genic regions, including 193 hyper- and 247 hypo-methylated DMRs, and may influence the expression of 137 downstream genes. Most hyper-methylated genes were associated with transferase activity, kinase activity, and phosphotransferase activity, whereas most hypo-methylated genes were associated with protein binding, ATP binding, and adenyl ribonucleotide binding, suggesting that different biological processes were activated during the day and night. Our results indicated that methylated genes were prevalent in the poplar genome, but that only a few of these participated in diurnal gene expression regulation.