文献类型：期刊文献

英文题名：Genome-wide identification of bHLH gene family and its response to cadmium stress in Populus x canescens

作者：Yao, Yuneng[1,2,3] He, Zhengquan[1] Li, Xinmeng[1,2,3] Xu, Jing[2,3] Han, Xiaojiao[2,3] Liang, Hongwei[1] Zhuo, Renying[2,3] Qiu, Wenmin[2,3]

第一作者：Yao, Yuneng

通信作者：Qiu, WM[1];Qiu, WM[2]

机构：[1]China Three Gorges Univ, Yichang, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou, Peoples R China;[3]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China

年份：2024

卷号：12

外文期刊名：PEERJ

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001335935400004)】；

基金：This work was supported by the National Nonprofit Institute Research Grant of CAF (No. RISF2021YZ01 and RISFZ-2021-01). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文关键词：bHLH gene family; Populus canescens; Cd stress

摘要：The basic helix-loop-helix (bHLH) gene family is integral to various aspects of plant development and the orchestration of stress response. This study focuses on the bHLH genes within Populus x canescens, a poplar species noted for its significant tolerance to cadmium (Cd) stress. Through our comprehensive genomic analysis, we have identified and characterized 170 bHLH genes within the P. canescens genome. These genes have been systematically classified into 22 distant subfamilies based on their evolutionary relationships. A notable conservation in gene structure and motif compositions were conserved across these subfamilies. Further analysis of the promoter regions of these genes revealed an abundance of essential cis-acting element, which are associated with plant hormonal regulation, development processes, and stress response pathway. Utilizing quantitative PCR (qPCR), we have documented the differential regulation of PcbHLHs in response to elevated Cd concentrations, with distinct expression patterns observed across various tissues. This study is poised to unravel the molecular mechanism underpinning Cd tolerance in P. canescens, offering valuable insights for the development of new cultivars with enhanced Cd accumulation capacity and tolerance. Such advancements are crucial for implementing effective phytoremediation strategies to mitigate soil pollution caused by Cd.