文献类型：期刊文献

英文题名：Unique features of the m(6)A methylome and its response to drought stress in sea buckthorn (Hippophae rhamnoides Linn.)

作者：Zhang, Guoyun[1,2] Lv, Zhongrui[1,2] Diao, Songfeng[3,4] Liu, Hong[1,2] Duan, Aiguo[1,2] He, Caiyun[1,2] Zhang, Jianguo[1,2,5]

第一作者：张国昀

通信作者：He, CY[1];Zhang, JG[1];He, CY[2];Zhang, JG[2]|[a0005b245645e47e67b16]何彩云;

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Natl Forestry & Grassland Adm, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat, Beijing, Peoples R China;[3]Chinese Acad Forestry, Nontimber Forestry Res & Dev Ctr, Zhengzhou, Peoples R China;[4]Natl Forestry & Grassland Adm, Key Lab Nontimber Forest Germplasm Enhancement &, Zhengzhou, Peoples R China;[5]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry South, Nanjing, Peoples R China

年份：2021

卷号：18

期号：S2

起止页码：794-803

外文期刊名：RNA BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000721136200001)】；

基金：This work was supported by the Fundamental Research Funds of CAF (CAFYBB2020SY006) and the Joint Funds of the National Natural Science Foundation of China (U2003116).

语种：英文

外文关键词：Sea buckthorn; N6-methyladenosine; drought stress; m(6)A modification; m(6)A demethylase

摘要：In plants, recent studies have revealed that N6-methyladenosine (m(6)A) methylation of mRNA has potential regulatory functions of this mRNA modification in many biological processes. m(6)A methyltransferase, m(6)A demethylase and m(6)A-binding proteins can cause differential phenotypes, indicating that m(6)A may have critical roles in the plant. In this study, we depicted the m(6)A map of sea buckthorn (Hippophae rhamnoides Linn.) transcriptome. Similar to A. thaliana, m(6)A sites of sea buckthorn transcriptome is significantly enriched around the stop codon and within 3MODIFIER LETTER PRIME-untranslated regions (3MODIFIER LETTER PRIMEUTR). Gene ontology analysis shows that the m(6)A modification genes are associated with metabolic biosynthesis. In addition, we identified 13,287 different m(6)A peaks (DMPs) between leaf under drought (TR) and control (CK) treatment. It reveals that m(6)A has a high level of conservation and has a positive correlation with mRNA abundance in plants. GO and KEGG enrichment results showed that DMP modification DEGs in TR were particularly associated with ABA biosynthesis. Interestingly, our results showed three m(6)A demethylase (HrALKBH10B, HrALKBH10C and HrALKBH10D) genes were significantly increased following drought stress, which indicated that it may contributed the decreased m(6)A levels. This exhaustive m(6)A map provides a basis and resource for the further functional study of mRNA m(6)A modification in abiotic stress.