文献类型：期刊文献

中文题名：HaNPV病毒对寄主昆虫的持续作用研究

英文题名：Studies on Sustainable Effects for Helicoverpa armigera Nuclear Polyhedrosis Virus to Host Insects

作者：曲良建[1] 张永安[1] 王玉珠[1] 郎杏茹[2]

第一作者：曲良建

机构：[1]中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室;[2]宁夏森林病虫害防治检疫总站

年份：2006

卷号：19

期号：2

起止页码：216-220

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家"十五"攻关项目"林木病虫害环境协调性农药及其航空喷洒技术研究"(2004BA509B12)

语种：中文

中文关键词：持续控制;棉铃虫核型多角体病毒;PCR

外文关键词：sustainable control; HaNPV; polymerase chain reaction（PCR）

分类号：S763

摘要：用不同浓度的HaNPV病毒感染3龄棉铃虫幼虫并分别收集不同处理的存活试虫进行室内传代饲养。结果表明,病毒对寄主昆虫不仅具有直接致死作用,而且对蛹质量和化蛹均有明显的影响,尤其是对亲代和子一代的寄主昆虫。同时,通过PCR技术,从子一代的幼虫和蛹中成功检测到该病毒,从而在分子水平上证明了病毒能够经过亲代传递到子代的种群中。这对病毒的高效利用和害虫的持续控制具有重要的理论和实践意义。
Third instars larvae of cotton bollworm, Helicoverpa armigera, were infected with different concentrations of Helicoverpa arrnigera nucleopolyhedrovirus （HaNPV）. Survivors were collected respectively and reared in laboratory room. Mortality rate of the larvae, average weight of the single pupa and pupation rate were observed in parental generation and successive filial generations. The results showed that the virus not only led to death of larvae in parental and folial generation, but also impacted the average weight of the single pupa and pupation rate, especially to the parental and the first folial generation. The experimental data indicated that the virus could weaken the host in folial generations, which could control populations of the host for a long time. Meanwhile, pelymerase chain reaction （PCR） amplification of polyhedrin gene sequences demonstrated that the virus was present in eggs and pupae whose parents were infected. This method might provide a better understanding of nucleopolyhedrovirus which could transmit from parental generation to folial generation. The research would play an important role in utilizing the virus effectively to control the pest persistently.