文献类型：期刊文献

英文题名：ABI5 modulates seed germination via feedback regulation of the expression of the PYR/PYL/RCAR ABA receptor genes

作者：Zhao, Hongyun[1] Nie, Kaili[1] Zhou, Huapeng[2] Yan, Xiaojing[3] Zhan, Qidi[1] Zheng, Yuan[1] Song, Chun-Peng[1]

第一作者：Zhao, Hongyun

通信作者：Zheng, Y[1]

机构：[1]Henan Univ, Sch Life Sci, State Key Lab Crop Stress Adaptat & Improvement, Kaifeng 475001, Peoples R China;[2]Sichuan Univ, Coll Life Sci, Key Lab Bioresource & Ecoenvironm, Minist Educ, Chengdu 610064, Peoples R China;[3]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2020

卷号：228

期号：2

起止页码：596-608

外文期刊名：NEW PHYTOLOGIST

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000545150500001)】；

基金：We thank Professor Yan Guo (State Key Laboratory of Plant Physiology and Biochemistry, China Agricultural University, Beijing) for providing guidance and giving valuable advice. This work was supported by the National Natural Science Foundation of China (grant nos. 31872656, 31870241 and 31600201).

语种：英文

外文关键词：ABI5; abscisic acid (ABA); feedback regulation; PYR1; PYL; RCARs; seed germination; transcriptional regulation

摘要：As abscisic acid (ABA) receptors, PYR1/PYL/RCAR (PYLs) play important roles in ABA-mediated seed germination, but the regulation of PYLs in this process, especially at the transcriptional level, remains unclear. In this study, we found that expression of 11 of 14PYLs changes significantly during seed germination and is affected by exogenous ABA. Two PYLs, PYL11 and PYL12, both of which are expressed specifically in mature seeds, positively modulate ABA-mediated seed germination. However, ABI5 was found to modulate the PYL11- and PYL12-mediated ABA response. In theabi5-7mutant, ABA hypersensitivity caused byPYL11andPYL12overexpression was totally or partially blocked. By contrast, ABI5 regulates the expression ofPYL11andPYL12by directly binding to their promoters. Moreover, the expression of eight otherPYLs is also affected during the germination ofabi5mutants. Promoter analysis revealed that an ABI5-binding region is present next to the TATA box or initiator box. Together, our data demonstrate the role of PYL11 and PYL12 in seed germination. In addition, the identification ofPYLs as targets of ABI5 reveals a role of ABI5 in the feedback regulation of ABA-mediated seed germination.