文献类型：期刊文献

英文题名：Moderate-density molecular maps of Eucalyptus urophylla S. T. Blake and E-tereticornis Smith genomes based on RAPD markers

作者：Gan S.[1] Shi J.[2] Li M.[1] Wu K.[1] Wu J.[1] Bai J.[1]

第一作者：甘四明

通信作者：Gan, SM[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Peoples R China;[2]Nanjing Forestry Univ, State Forestry Adv, Key Lab Forest Tree Genet & Gene Engn, Nanjing 210037, Peoples R China

年份：2003

卷号：118

期号：1

起止页码：59-67

外文期刊名：GENETICA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000181764800008)】；

基金：This work was supported in part by grants from the National Natural Science Foundation of China (NSFC Grant 39870619) and the International Foundation for Science to SG (IFS Grant D/2947-1). Thanks are given to Prof. Mingxiu Wang, Prof. Minren Huang, Dr Tongming Yin, and Dr Zhenxiang He for valuable advice and technical assistance as well as Dr Simon Southerton, Dr Penny Butcher and the two anonymous reviewers for their critical comments on the manuscript.

语种：英文

外文关键词：Eucalyptus tereticornis; Eucalyptus urophylla; genome; linkage map; RAPD

摘要：Moderate-density molecular maps were constructed for the genomes of Eucalyptus urophylla S. T. Blake and E. tereticornis Smith using RAPD markers and an interspecific cross between the two species. One hundred and eighty-three primers were employed to generate 245 and 264 parent-specific markers in E. urophylla and E. tereticornis, respectively, as well as 49 parent-shared markers. The normally segregating markers, including 208 (84.9%) specific to maternal E. urophylla, 175 (66.3%) to paternal E. tereticornis, and 48 shared by both parents, were used for framework map construction for each parental species. For maternal E. urophylla, the linkage map consisted of 23 linkage groups, 160 framework markers, and 60 accessory markers, defining a total map distance of 1504.6 cM and an average interval of 11.0 +/- 8.07 cM. For paternal E. tereticornis, the linkage map contained 23 linkage groups, 126 framework markers, and 92 accessory markers, defining a total map distance of 1035.7 cM and an average interval of 10.1 +/- 7.23 cM. Genome length was estimated at 1585.7 and 1507.5 cM for E. urophylla and E. tereticornis, respectively, indicating map coverage of 94.9 and 68.7% of the corresponding genomes. Construction of such maps will be valuable for quantitative trait loci (QTLs) detection, marker-assisted selection (MAS), comparative mapping, and whole genome based fingerprint characterization in Eucalyptus breeding programs.