文献类型：期刊文献

英文题名：Sir2 family proteins regulate terpenoid synthesis by deacetylation of 3-hydroxy-3-methylglutaryl-CoA synthase

作者：Wu, Liwen[1,2] Chen, Yicun[1,2] Gao, Ming[1,2] Zhao, Yunxiao[1,2] Wang, Yangdong[1,2]

第一作者：吴立文

通信作者：Wang, YD[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China

年份：2021

卷号：170

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20212710578308);Scopus(收录号：2-s2.0-85108896682);WOS:【SCI-EXPANDED(收录号:WOS:000690967600003)】；

基金：We acknowledge the support received through the Fundamental Research Funds of Chinese Academy of Forestry, China (No. CAFYBB2019ZX002).

语种：英文

外文关键词：Sir2; Terpenoid; Deacetylation; HMGS; Litsea cubeba

摘要：Terpenoids are the main components of plant essential oils, and each stage of their synthesis pathway has been identified. However, there are few reports on the regulatory mechanism of this synthesis pathway. Our previous research showed that increasing the gene expression level of one of the key rate-limiting enzymes in the pathway, 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS), can promote this synthesis pathway. In this study, we cloned Sir2 deacetylase family genes in the essential oil-producing plant Litsea cubeba, analysed their functions, and found that overexpression of this gene family can also effectively increase the type and production of terpenoids. In addition, overexpression of this gene family induced phenotypes such as excessive growth and enlarged vegetative organs in plants. Subsequently, we analysed the regulatory mechanism of the Sir2 family and found that these proteins can increase the enzymatic activity of HMGS through deacetylation, thereby promoting the synthesis of terpenoids as the final product. This study discovered a posttranslational regulatory mechanism in the terpenoid synthesis pathway. In addition, this study also provides an effective metabolic engineering method to increase the yield of plant terpenoids while increasing the biomass of plants.