文献类型：期刊文献

中文题名：FISH-AFLP分析新疆核桃品种遗传多样性

英文题名：Genetic Diversity on Walnut Cultivars in Xinjiang Revealed by FISH-AFLP

作者：何健[1,2] 马庆国[2] 雪来提·牙生[1] 孙东[1] 孙世国[3] 裴东[2]

第一作者：何健

机构：[1]喀什地区林业技术推广站;[2]中国林业科学研究院林业研究所,林木遗传育种国家重点实验室,国家林业局林木培育重点实验室;[3]阿克苏地区林业科学研究所

年份：2013

卷号：26

期号：6

起止页码：730-735

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家‘十二五’科技支撑计划"核桃和长山核桃高效生产关键技术研究与示范"(2013BAD14B01);国家自然科学基金项目"核桃埋干复幼促根过程中生长素响应基因的CpG岛甲基化重编程研究"(31171933)

语种：中文

中文关键词：核桃;品种;遗传多样性;FISH—AFLP;亲缘关系

外文关键词：Juglans regia L. ; cultivar; genetic diversity; FISH-AFLP; sibship

分类号：S792.13

摘要：采用FISH-AFLP技术,选取8对EcoRⅠ+3和MseⅠ+3引物组合,对新疆维吾尔自治区林木品种审定委员会审(认)定的24个核桃品种(无性系)、2株实生古树及4个农家类型在DNA水平上进行了多态性检测。结果表明:在获得的1 011条谱带中,981条呈多态性,多态性百分率达97.5%;不同引物组合的有效等位基因数为1.188 71.234 7,平均为1.208 5;基因多样度为0.118 3 0.141 2,平均为0.129 7;Shannon信息指数为0.184 6 0.225 8,平均为0.206 6。总体的遗传多样性水平为中等。经8对引物检测的30个品种及类型均得到数目不等的特异带型,能将30个核桃良种及类型完全区分,建立了它们的指纹图谱。
In order to view the nut cultivars （Juglans regia L. netic basis of Xinjiang walnut germplasm resourses, the genetic diversity of 30 wal- authorized or approved by Xinjiang Uygur Autonomous Region Forest Tree Cuhivar Registration Committee were investigated by using FISH-AFLP. Eight pairs of EcoR I ＋ 5/Mse I ＋ 3 primer combi- nations were used to amplify the genomic DNA. 981 out of totally 1011 AFLP bands were polymorphic and the aver- age percent of polymorphic bands （PPB） was 97.5 %. The value of effective number of alleles （Ne） estimated by different primer combinations ranged from 1. 188 7 to 1. 234 7 with an average of 1. 208 5 ; Nei＇ s gene diversity （H） ranged from 0. 118 3 to 0. 141 2 with an average of 0. 129 7 and Shannon＇ s information index （I） ranged from 0. 184 6 to 0. 225 8 with an average of 0. 206 6, which revealed a moderate level of genetic diversity. Unique finger- printings for 30 walnut cultivars were established by 8 primer combinations in the research.