文献类型：期刊文献

英文题名：XsLTPG31 Confers Leaf Cuticular Wax Deposition and Drought Resistance in Yellowhorn

作者：Xu, Huihui[1] Liu, Xiaojuan[1] Ban, Zhuo[1] Yang, Yingying[1] Zhang, Lingfeng[1] Bi, Quanxin[1] Wang, Libing[1]

第一作者：Xu, Huihui

通信作者：Wang, LB[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China

年份：2025

外文期刊名：PLANT CELL AND ENVIRONMENT

收录：;Scopus(收录号：2-s2.0-105014095179);WOS:【SCI-EXPANDED(收录号:WOS:001556133800001)】；

基金：This study was supported by the Central Public-Interest Scientific Institution Basal Research Fund (CAFYBB2023MB002) and the National Natural Science Foundation of China (32271838, 31901348).

语种：英文

外文关键词：cuticular wax; drought resistance; genome-wide association studies; leaf anatomical structural traits; XsLTPG31

摘要：With increasing frequency and severity under climate change, drought stress has become a predominant abiotic limiting factor of global plant productivity. Yellowhorn (Xanthoceras sorbifolium Bunge), an endemic species of woody oil tree in North China, has substantial developmental potential in arid and semi-arid regions. To elucidate the genetic basis of its drought response, a genome-wide association study (GWAS) of 13 leaf anatomical structure traits across 237 yellowhorn accessions was performed in this study, and 21 candidate genes that regulate leaf structural variation were identified. Among these genes, XsLTPG31, which encodes a nonspecific lipid transfer protein, was significantly induced by drought stress. Heterologous overexpression of XsLTPG31 in Arabidopsis, its transient overexpression in yellowhorn leaves, and virus-induced gene silencing (VIGS) of XsLTPG31 demonstrated that XsLTPG31 promotes the deposition of leaf epidermal wax and modulates drought resistance through facilitating the export of wax to the extracellular space. Moreover, we revealed that XsLTPG31 is directly activated by XsMYB16 via promoter binding. Taken together, the results of our study enhance the understanding of the regulatory mechanisms underlying LTPG-mediated cuticular wax deposition and might provide targets for the breeding of drought-tolerant varieties of yellowhorn.