文献类型：期刊文献

中文题名：春石斛的多倍体诱导

英文题名：Polyploid Induction of Nobile-type Dendrobium

作者：郑宝强[1] 张莹[1,2] 王雁[1] 李振坚[1] 朱向涛[1] 律春燕[1]

第一作者：郑宝强

机构：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点试验室;[2]中国林业科学院亚热带林业研究所

年份：2009

期号：9

起止页码：1381-1384

中文期刊名：园艺学报

外文期刊名：Acta Horticulturae Sinica

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家林业局‘948’项目(2006-4-C07;2005-4-37)

语种：中文

中文关键词：石斛;春石斛;多倍体;秋水仙素;染色体

外文关键词：Dendrobium ; nobile-type Dendrobium ; polyploid ; colchicine ; chromosome

分类号：S682.31

摘要：在离体培养条件下比较了秋水仙素不同浓度、不同处理时间诱导杂种春石斛体细胞染色体加倍的效果。结果表明,0.06%秋水仙素处理12h的诱变效果最佳,诱变率达69.1%。用组织培养结合不定芽诱导技术获得了稳定的多倍体变异株。变异株与正常株形态差异明显,气孔数及大小之间差异显著。二倍体对照2n=2x=38,变异株2n=4x=76(四倍体),同时也发现存在嵌合体现象。
A hybrid of nobile-type Dendrobium was treated with colchicines at different concentration and time duration in vitro. The results showed that the best effect was obtained by the treatment of 0.06% colchicines with 12 hours and its induction rate was 69. 1%. Comparing the characteristics of plants and stomas, there were remarkable difference between the treatment and the control. Chromosome observation found that the chromosome number of treated plants was 76 and the chromosome number of the control was 38, indicating the tetraploid （2n = 4x = 76） of nobile-type Dendrobium was induced successfully. The mixer-fold phenomenon was also found among treatment plants.