文献类型：期刊文献

英文题名：Copper stress in flooded soil: Impact on enzyme activities, microbial community composition and diversity in the rhizosphere of Salix integra

作者：Cao, Yini[1] Ma, Chuanxin[2] Chen, Hongjun[3] Chen, Guangcai[1] White, Jason C.[2] Xing, Baoshan[4]

第一作者：Cao, Yini

通信作者：Chen, GC[1]|[a000551c9abd7c3960d59]陈光才;

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China;[2]Connecticut Agr Expt Stn, Dept Analyt Chem, New Haven, CT 06504 USA;[3]Hunan Commod Qual Supervis & Inspect Inst, Changsha 410007, Hunan, Peoples R China;[4]Univ Massachusetts, Stockbridge Sch Agr, Amherst, MA 01003 USA

年份：2020

卷号：704

外文期刊名：SCIENCE OF THE TOTAL ENVIRONMENT

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000504672800031)】；

基金：The work was supported by the Key Research and Development Program of Zhejiang Province, China (2018C03047) and the National Natural Science Foundation of China (31470619).

语种：英文

外文关键词：Heavy metal; Flooding; Rhizo sphere; Microflora; Enzyme activity

摘要：Climate change has increased flooding frequency, making the heavy metal polluted areas more vulnerable, and led to increased global land degradation. Information about the alteration of soil microbiota under heavy metal pollution and flooding is still rather limited. Fast-growing trees are candidates for phytoremediation of heavy metal polluted soils. Therefore, the impact of Cu pollution on microbiota in soil used for cultivating Salix integra Thunb. was investigated with and without flooding for 60 d. Bacterial and fungal communities were accessed via partial 16S rRNA (V-3-V-4) and internal transcribed spacer (ITS) genes. The activity of invertase, urease and cellulase were markedly decreased by 28.5-59%, 55.0-76.7% and 17.3-34.1%, respectively, with increasing Cu levels. Flooding significantly increased the activity of polyphenol oxidase and peroxidase by 56.3% and 41.4% at the highest Cu level compared to its respective nonflooded condition. High Cu concentration significantly decreased the richness and diversity of the bacterial community, and fungi were more sensitive than bacteria under flooding conditions. Redundancy analysis suggests that Cu, Fe and soil organic matter are the key determinants affecting the composition of microbial communities. Our findings provide new insight into the responses of soil microbes to Cu-contamination and contribute to our understanding of metal toxicity in soil-woody plant systems under flooded conditions. (C) 2019 Elsevier B.V. All rights reserved.