文献类型：期刊文献

英文题名：Large-scale evidence for microbial response and associated carbon release after permafrost thaw

作者：Chen, Yongliang[1,2] Liu, Futing[1,3] Kang, Luyao[1,4] Zhang, Dianye[1] Kou, Dan[1] Mao, Chao[1,4] Qin, Shuqi[1,4] Zhang, Qiwen[1] Yang, Yuanhe[1,4]

第一作者：Chen, Yongliang

通信作者：Yang, YH[1]

机构：[1]Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China;[2]China Agr Univ, Coll Resources & Environm Sci, Beijing, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat, State Forestry & Grassland Adm, Beijing, Peoples R China;[4]Univ Chinese Acad Sci, Beijing, Peoples R China

年份：2021

卷号：27

期号：14

起止页码：3218-3229

外文期刊名：GLOBAL CHANGE BIOLOGY

收录：;Scopus(收录号：2-s2.0-85100134477);WOS:【SCI-EXPANDED(收录号:WOS:000609278300001)】；

基金：National Key Research and Development Program of China, Grant/Award Number: 2017YFA0604803 and 2016YFC0500701; National Natural Science Foundation of China, Grant/Award Number: 31825006, 31988102 and 91837312; Second Tibetan Plateau Scientific Expedition and Research, Grant/Award Number: 2019QZKK0106

语种：英文

外文关键词：carbon cycle; climate warming; GeoChip; Illumina sequencing; microbial community; permafrost thaw

摘要：Permafrost thaw could trigger the release of greenhouse gases through microbial decomposition of the large quantities of carbon (C) stored within frozen soils. However, accurate evaluation of soil C emissions from thawing permafrost is still a big challenge, partly due to our inadequate understanding about the response of microbial communities and their linkage with soil C release upon permafrost thaw. Based on a large-scale permafrost sampling across 24 sites on the Tibetan Plateau, we employed meta-genomic technologies (GeoChip and Illumina MiSeq sequencing) to explore the impacts of permafrost thaw (permafrost samples were incubated for 11 days at 5 degrees C) on microbial taxonomic and functional communities, and then conducted a laboratory incubation to investigate the linkage of microbial taxonomic and functional diversity with soil C release after permafrost thaw. We found that bacterial and fungal alpha diversity decreased, but functional gene diversity and the normalized relative abundance of C degradation genes increased after permafrost thaw, reflecting the rapid microbial response to permafrost thaw. Moreover, both the microbial taxonomic and functional community structures differed between the thawed permafrost and formerly frozen soils. Furthermore, soil C release rate over five month incubation was associated with microbial functional diversity and C degradation gene abundances. By contrast, neither microbial taxonomic diversity nor community structure exhibited any significant effects on soil C release over the incubation period. These findings demonstrate that permafrost thaw could accelerate C emissions by altering the function potentials of microbial communities rather than taxonomic diversity, highlighting the crucial role of microbial functional genes in mediating the responses of permafrost C cycle to climate warming.