文献类型：期刊文献

英文题名：Integrated Transcriptome and Metabolome Analysis Revealed the Key Role of the Flavonoid Biosynthesis in Olive Defense Against Alternaria alternata

作者：Wang, Tianyi[1,2] Cao, Hongyan[3] Du, Tingting[3] Meng, Dong[3] Yang, Qing[3] Li, Jinhua[1,2] Zhang, Jianguo[1,2] Zeng, Yanfei[1,2]

第一作者：Wang, Tianyi

通信作者：Zhang, JG[1];Zeng, YF[1];Zhang, JG[2];Zeng, YF[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm, Beijing, Peoples R China;[3]Beijing Forestry Univ, Key Lab Silviculture & Conservat, Minist Educ, Beijing, Peoples R China

年份：2024

卷号：176

期号：5

外文期刊名：PHYSIOLOGIA PLANTARUM

收录：;Scopus(收录号：2-s2.0-85204941030);WOS:【SCI-EXPANDED(收录号:WOS:001319929800001)】；

基金：This work was funded by the National Key R&D Program of China (2023YFD2200300; 2019YFD1000600). We thank Dr. Wanfeng Li for his useful comments on this paper.

语种：英文

摘要：The olive tree is an important oil woody plant with high economic value, yet it is vulnerable to the attack of numerous fungi. The successful control of olive fungal diseases requires a comprehensive understanding of the disease resistance mechanisms in plants. Here, we isolated Alternaria alternata from the diseased leaves of olive plants, and screened a resistant ("Leccino") and susceptible ("Manzanilla de Sevilla") cultivar from eight olive cultivars to explore their resistance mechanisms. Transcriptomic and metabolomic analyses identified the flavonoid biosynthesis as a key defense pathway against A. alternata. Five important transcription factors associated with flavonoid biosynthesis were also determined. The overexpression of OeWRKY40 significantly enhanced the disease resistance of the susceptible cultivar and upregulated the expression of genes involved in flavonoid biosynthesis and the accumulation of related metabolites. LUC assays further proved that OeWRKY40 can activate the expression of OeC4H. These results help to better clarify the molecular mechanisms of flavonoid biosynthesis against A. alternata. Our study provides key information for further exploration of the molecular pathways of olive plants and their resistance to fungi, an important factor for molecular breeding and utilization of resistant cultivars.