文献类型：期刊文献

英文题名：Isolation and characterization of microsatellite loci from an endangered tree species, Toona ciliata var. pubescens

作者：Liu, J.[1] Sun, Z. -X.[2] Chen, Y. -T.[1] Jiang, J. -M.[1]

第一作者：刘军

通信作者：Liu, J[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou, Zhejiang, Peoples R China;[2]China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou, Zhejiang, Peoples R China

年份：2012

卷号：11

期号：4

起止页码：4411-4417

外文期刊名：GENETICS AND MOLECULAR RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000313960500085)】；

基金：Research supported by the National Natural Science Foundation Project (Grant #31100487) and the National Forestry Public Welfare Industry Research Project (Grant #201104001). We sincerely thank Hu Guocheng, Liu Wenzhen, Luan Weijiang, and Zhuo Renying for their help. We also thank He Guiping, Wu Tianlin, and Li Jiangchuan for the collection of samples.

语种：英文

外文关键词：Microsatellite loci; Endangered tree; Isolation

摘要：Toona ciliata var. pubescens is considered an endangered tree species native to China. In order to help develop a conservation program for this species, we evaluated its genetic diversity and population genetics. We isolated microsatellite DNA loci using streptavidin beads. A genomic library, enriched with microsatellites, was constructed and screened by sequencing. We detected 8 polymorphic microsatellite loci from the tree tissue samples. The population of T. ciliata var. pubescens used in this study is located within the Guanshan National Nature Reserve, Jiangxi Province, China. Sixty-five individuals were collected for the study. The Guanshan population was split into two subpopulations due to terrain. The number of alleles per locus ranged from 2 to 6, with expected heterozygosity from 0.2386 to 0.6772. Four of the 8 loci, except loci Tc02, Tc04, Tc05, and Tc07 showed no significant departure from Hardy-Weinberg equilibrium. The mean observed heterozygosity was 0.59. The average coefficient of genetic differentiation between the two subpopulations was quite low (F-ST = 0.0235). The level of gene flow (N-m) was 10.39, reflecting a high degree of gene flow between the two subpopulations.