文献类型：期刊文献

英文题名：Melatonin improves the survival of cryopreserved callus of Rhodiola crenulata

作者：Zhao, Yan[1,2] Qi, Li-Wang[3] Wang, Wei-Ming[4] Saxena, Praveen K.[5] Liu, Chun-Zhao[1,2]

第一作者：Zhao, Yan

通信作者：Liu, CZ[1]

机构：[1]Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China;[2]Chinese Acad Sci, Grad Sch, Beijing 100190, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, Beijing, Peoples R China;[4]Phyton Biotech Inc, E Windsor, NJ USA;[5]Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada

年份：2011

卷号：50

期号：1

起止页码：83-88

外文期刊名：JOURNAL OF PINEAL RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000285154400011)】；

基金：This work was funded by the National High Technology Research and Development Program (863 Program) of China (No. 2007AA021501), the Knowledge Innovation Program of the Chinese Academy of Sciences (No.YZ-06-03), and the Natural Sciences and Engineering Research Council of Canada.

语种：英文

外文关键词：antioxidative enzymes; cryopreservation; malondialdehyde; melatonin; Rhodiola crenulata

摘要：An important aspect of the function of melatonin seems to be the mediation of stress caused by environmental and chemical factors. In the cryopreservation process, environmental changes including osmotic injury, desiccation, and low temperature can impose a series of stresses on plants. In this study, we evaluated the role of melatonin in stress protection during the process of cryopreservation using callus of an endangered plant species Rhodiola crenulata. The survival rate of the cryopreserved callus significantly increased when the callus was pretreated for 5 days with 0.1 mu m melatonin prior to freezing in liquid nitrogen. Analysis of antioxidative activity following the pretreatment of callus with 0.1 mu m melatonin showed a significant reduction in malondialdehyde production during various steps of cryopreservation. Enhanced peroxidase and catalase activity was observed in the callus after pretreatment with 0.1 mu m melatonin compared to the control. These observations provide new evidence of the antioxidant/anti-stress function of melatonin, and it is the first report of its potential application in the preservation of elite endangered germplasm through the process of cryopreservation.