文献类型：期刊文献

英文题名：Proteomic analysis and candidate allergenic proteins in Populus deltoides CL. "2KEN8" mature pollen

作者：Zhang, Jin[1,2,3] Wu, Li-Shuan[1,4,5] Fan, Wei[1] Zhang, Xiao-Ling[1] Jia, Hui-Xia[1,2] Li, Yu[1] Yin, Ya-Fang[3] Hu, Jian-Jun[1,2] Lu, Meng-Zhu[1,2]

第一作者：Zhang, Jin

通信作者：Hu, JJ[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry A, Res Inst Forestry, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing, Jiangsu, Peoples R China;[3]Chinese Acad Forestry, Res Inst Wood Ind, Beijing, Peoples R China;[4]Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China;[5]Univ Chinese Acad Sci, Beijing, Peoples R China

年份：2015

卷号：6

期号：JULY

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000358726300002)】；

基金：This work was supported by Special Fund for Forestry Scientific Research in the Public Interest [201304103-4] and National High-tech R&D Program of China [2011AA100201] to JH. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文关键词：allergen; MALDI-TOF/TOF MS/MS; pollen; Populus deltoids; profilin; proteomics; two-dimensional gel electrophoresis

摘要：Proteomic analysis was used to generate a map of Populus deltoides CL. "2KEN8" mature pollen proteins. By applying 2-D electrophoresis, we resolved 403 protein spots from mature pollen. Using the matrix-assisted laser desorption/ionization time time-of-flight/time-of-flight tandem mass spectrometry method, we identified 178 distinct proteins from 218 protein spots expressed in mature pollen. Moreover, out of these, 28 proteins were identified as putative allergens. The expression patterns of these putative allergen genes indicate that several of these genes are highly expressed in pollen. In addition, the members of profilin allergen family were analyzed and their expression patterns were compared with their homologous genes in Arabidopsis and rice. Knowledge of these identified allergens has the potential to improve specific diagnosis and allergen immunotherapy treatment for patients with poplar pollen allergy.