文献类型：期刊文献

中文题名：M13分子标记快速区分树木溃疡病原真菌的种类

英文题名：M13 molecular marker technique rapidly distinguish pathogenic fungal group causing tree canker

作者：王庆灵[1,2] 崔建州[1] 赵嘉平[2]

第一作者：王庆灵

机构：[1]河北农业大学林学院,河北保定071001;[2]中国林业科学研究院林业新技术研究所,遗传育种国家重点开放试验室,北京100091

年份：2013

期号：1

起止页码：74-79

中文期刊名：河北农业大学学报

外文期刊名：Journal of Agricultural University of Hebei

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD_E2013_2014】；

基金：科技基础性工作专项“中国树木溃疡病原多样性及其生态地理分布和危害调查”(2009FY210100)

语种：中文

中文关键词：树木溃疡病;引物M13;分子标记;rDNA内转录间隔区

外文关键词：tree canker; primer M13; molecular marker technique; rDNA-ITS

分类号：S763.1

摘要：树木溃疡病菌具有寄主多样性、危害症状复杂且分布广泛的特点,快速简便地区分真菌类别是杨树溃疡病研究及防治中需要解决的重要问题。在对树木溃疡病菌的遗传多样性研究,发现以M13为引物的PCR扩增可以在不同的种类之间产生稳定分化的带型。因此,本研究分离了43株18种树木溃疡病相关真菌,并且对这些菌株进行rDNA-ITS序列测定及M13-PCR。结果显示,M13带型差异与真菌的特定种类相对应。因此,认为M13分子标记可以准确快速地鉴定、区分树木溃疡病菌。
Pathogenic fungal group causing tree canker has a variety of hosts , complex harm syptom and a wide distribution. Simply and conveniently identifying fungal group is an impor- tant problem in the study of prevention and control of tree canker. During studying genetic di- versity of tree caker pathogens, primer M13 PCR produced stable and differential bands in dif- ferential species. Therefore, to verify the differential ability of M13, this study separated 43 i- solates of tree caker related fungi , which were determined by rDNA-ITS sequences and M13- PCR of the isolates. The result proved that M13 band diversity was corresponds with specific species. The results suggest that M13 molecular marker technique can distinguish different can- ker pathogens accurately and rapidly.