详细信息
广东车八岭自然保护区疑似黑熊爪鞘的分子鉴定
Molecular Identification of Suspected Claw Sheath from Ursus thibetanus in Chebaling National Nature Reserve,Guangdong Province
文献类型:期刊文献
中文题名:广东车八岭自然保护区疑似黑熊爪鞘的分子鉴定
英文题名:Molecular Identification of Suspected Claw Sheath from Ursus thibetanus in Chebaling National Nature Reserve,Guangdong Province
作者:廖丽晶[1] 金崑[1] 胡慧建[2] 张于光[1] 张亮[2]
第一作者:廖丽晶
机构:[1]中国林业科学研究院森林生态环境与保护研究所;[2]华南濒危动物研究所
年份:2011
卷号:39
期号:11
起止页码:105-107
中文期刊名:东北林业大学学报
外文期刊名:Journal of Northeast Forestry University
收录:CSTPCD;;北大核心:【北大核心2008】;CSCD:【CSCD2011_2012】;
语种:中文
中文关键词:亚洲黑熊;线粒体Cyt;b基因;物种鉴定;车八岭国家级自然保护区
外文关键词:Ursus thibetanus; Cyt b gene; Species identification; Chebaling national nature reserve
分类号:Q97
摘要:利用食肉目线粒体Cyt b基因的特异性引物对2010年4月在广东车八岭自然保护区内发现的爪鞘进行了分子鉴定。所测序列与亚洲黑熊(Ursus thibetanus)的同源性最高,达97.2%~99.1%,确定爪鞘来自于亚洲黑熊,为近20 a来首次在车八岭保护区发现的亚洲黑熊存在痕迹。
DNA was direcdy extracted from a claw sheath found in Chebaling National Nature Reserve, then the mtDNA Cyt b gene fragment was amplified and sequenced. Sequences were aligned with the GenBank by BLAST software, which showed the highest homology to the sequences from the black bear ( Ursus thibetanus) , reaching 97.2%-99. 1%. The claw sheath was identified from U. thibetanus. It is the first time we have discovered trace of U. thibetanus appeared in Chebaling National Nature Reserve in recent 20 years.
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