文献类型：期刊文献

英文题名：Physcion, a natural anthraquinone derivative, enhances the gene expression of leaf-specific thionin of barley against Blumeria graminis

作者：Ma, Xingxia[1,2] Yang, Xiaojun[2,3] Zeng, Fansong[3] Yang, Lijun[3] Yu, Dazhao[3] Ni, Hanwen[1]

第一作者：马星霞;Ma, Xingxia

通信作者：Ni, HW[1]

机构：[1]China Agr Univ, Coll Agron & Biotechnol, Beijing 100193, Peoples R China;[2]Chinese Acad Forestry, Res Inst Wood Ind, Beijing, Peoples R China;[3]Hubei Acad Agr Sci, Inst Plant Protect & Soil Sci, Wuhan, Peoples R China

年份：2010

卷号：66

期号：7

起止页码：718-724

外文期刊名：PEST MANAGEMENT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000279129300005)】；

基金：This research was supported by the China National Natural Science Foundation (30971955) and a grant from the China National Key Project of the 11th Five-Year Plan (2006BAD19B04).

语种：英文

外文关键词：physcion; thionin; defence-related genes; Blumeria graminis; barley

摘要：BACKGROUND: Physcion is a key active ingredient of the ethanol extract from roots of Chinese rhubarb (Rheum officinale Baill.) that has been commercialised in China for controlling powdery mildews. The biological mechanism of action of physcion against the barley powdery mildew pathogen was studied using bioassay and microarray methods. RESULTS: Bioassay indicated that physcion did not directly affect conidial germination of Blumeria graminis Speer f. sp. hordei Marchel, but significantly inhibited conidial germination in vivo. Challenge inoculation indicated that physcion induced localised resistance rather than systemic resistance against powdery mildew. Gene expression profiling of physcion-treated barley leaves detected four upregulated and five downregulated genes (ratio >= 2.0 and P-value <0.05) by using an Affymetrix Barley GeneChip. The five upregulated probe sequences blasted to the same barley leaf-specific thionin gene, with significant changes varying from 4.26 to 19.91-fold. All downregulated genes were defence-related, linked to peroxidase, oxalate oxidase, bsi1 protein and a pathogenesis-related protein. These changes varied from -2.34 to -2.96. Quantitative real-time PCR data confirmed that physcion enhanced the gene expression of leaf-specific thionin of barley. CONCLUSION: Results indicated that physcion controls powdery mildew mainly through changing the expression of defence-related genes, and especially enhancing expression of leaf-specific thionin in barley leaves. (C) 2010 Society of Chemical Industry