文献类型：期刊文献

中文题名：3-溴丙酮酸对美国白蛾核型多角体病毒的增效作用

英文题名：Synergistic Effect of 3-bromopyruvate Combined with Hyphantria cunea Nucleopolyhedrovirus Against Hyphantria cunea(Drury)

作者：刘瑞霞[1,2] 李恩杰[3] 柏超[4] 王青华[1] 段立清[2]

第一作者：刘瑞霞

机构：[1]中国林业科学研究院森林生态环境与自然保护研究所/国家林业和草原局森林保护学重点实验室,北京100091;[2]内蒙古农业大学林学院,内蒙古呼和浩特010019;[3]中国林业科学研究院华北林业实验中心,北京102300;[4]北京市新海园林工程有限公司,北京100018

年份：2022

卷号：35

期号：6

起止页码：101-107

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：中国林科院中央级公益性科研院所基本科研业务费专项基金(CAFYBB2018QB005);十三五国家重点研发计划(2018YFD0600202)。

语种：中文

中文关键词：美国白蛾核型多角体病毒;3-溴丙酮酸;美国白蛾;增效作用

外文关键词：Hyphantria cunea nucleopolyhedrovirus;3-bromopyruvate;Hyphantria cunea(Drury);Synergistic effect

分类号：S767.3

摘要：[目的]利用3-溴丙酮酸(3-BrPA)作为美国白蛾核型多角体病毒(HycuNPV)的生物增效剂,探讨3-BrPA对HycuNPV的毒力和对美国白蛾生长发育的影响,为进一步推进HycuNPV杀虫剂的应用提供新的增效剂。[方法]通过室内在HycuNPV中添加不同剂量3-BrPA,分析其对HycuNPV的增效作用以及对美国白蛾生长发育的影响。[结果]室内生物活性结果表明,3-BrPA对HycuNPV具有增效作用,不仅可以提高HycuNPV的感染能力,还能提高杀虫速度。当3-BrPA的添加剂量为5.0、10.0和15.0 mg·g^(?1)时,HycuNPV作用于美国白蛾3龄幼虫的LC_(50)都有不同程度地降低;并且对HycuNPV的增效倍数(S_(R))分别为825.14、164.72和0.47,共毒系数(CTC)分别为82613.63、16579.25和147.27。不同浓度的HycuNPV在不同剂量的3-BrPA作用下,其杀虫效果各不相同。添加不同剂量的3-BrPA,LT_(50)缩短了3.280~9.724 d。根据SR、CTC、LT_(50)及ST等指标评判,在浓度为9.0×10^(5)OBs·mL^(?1)的HycuNPV中添加5.0 mg·g^(?1)的3-BrPA时,增效作用最优。此外,3-BrPA和HycuNPV联合作用还显著抑制了幼虫的生长发育,且存活幼虫的化蛹率和羽化率均低于单独使用病毒杀虫剂。[结论]3-BrPA对HycuNPV具有增效作用,有望作为理想的病毒杀虫剂的增效剂。
[Objective]In this paper,we used 3-bromopyruvate(3-BrPA)as a biological synergist of Hyphantria cunea nucleopolyhedrovirus(HycuNPV)to investigate the effect of 3-BrPA on the virulence of HycuNPV and the growth and development of Hyphantria cunea,which will provide new synergist resources for the application of HycuNPV insecticides.[Method]The synergistic effect on HycuNPV was analyzed by adding different doses of 3-BrPA incorporating with HycuNPV in laboratory.[Result]The bioassay results showed that 3-BrPA had a synergistic effect on HycuNPV.It could not only improve infectivity of HycuNPV,but also increase the insecticidal speed.When the dosages of 3-BrPA with 5.0,10.0 and 15.0 mg·g^(?1) were added to HycuNPV,the LC_(50) of HycuNPV in the 3^(rd) instar larvae of H.cunea decreased.The synergistic ratio(SR)of HycuNPV were 825.14,164.72 and 0.47,and the co-toxicity coefficients(CTC)were 82613.63,16579.25 and 147.27,respectively.Different concentrations of HycuNPV had different insecticidal effects under the different doses of 3-BrPA.Added different doses of 3-BrPA,the LT_(50) of HycuNPV against the larvae were shorten by 3.280~9.724 d.According to S_(R),CTC,LT_(50) and ST,the optimum synergistic effect was observed:5.0 mg·g^(?1)3-BrPA+9.0×10^(5) OBs·mL^(?1) HycuNPV.In addition,the combination of 3-BrPA and HycuNPV significantly inhibited the growth and development of larvae,and the pupation and emergence rate of surviving larvae were significantly lower than that of the virus insecticide alone.[Conclusion]3-BrPA has a synergistic effect on HycuNPV,and is expected to be an ideal synergistic resource of viral insecticides.