文献类型：期刊文献

英文题名：Poplar PsnICE1 enhances cold tolerance by binding to different cis-acting elements to improve reactive oxygen species-scavenging capability

作者：Wang, Yan-Min[1,2,3] Zhang, Yi-Ming[2] Zhang, Xin[2] Zhao, Xin[2] Zhang, Yu[1] Wang, Chao[2] Wang, Yu-Cheng[2,4] Wang, Liu-Qiang[1]

第一作者：Wang, Yan-Min

通信作者：Wang, LQ[1];Wang, YC[2];Wang, YC[3]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, State Forestry Adm,Res Inst Forestry, Dongxiaofu 1, Beijing 100091, Peoples R China;[2]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, 51 Hexing Rd, Harbin 150040, Peoples R China;[3]Forestry Sci Res Inst Heilongjiang Prov, Key Lab Fast Growing Tree Cultivating Heilongjian, 134 Haping Rd, Harbin 150081, Peoples R China;[4]Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, 818 Beijingnan Rd, Urumqi 830011, Peoples R China

年份：2021

卷号：41

期号：12

起止页码：2424-2437

外文期刊名：TREE PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000742398400015)】；

基金：This work was supported by the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (no. CAFYBB2018QB002), National Natural Science Foundation of China (no. 31770704) and 111 project of China (no. B16010).

语种：英文

外文关键词：cis-acting element; cold tolerance; ICE1 transcription factor; Populus simonii x P. nigra; reactive oxygen species

摘要：Low temperature is a major stress that severely affects plant growth and development. Inducer of CBF expression 1 (ICE1) plays a key role in plant cold tolerance by regulating the expression of cold stress-responsive genes. In the present study, we characterized the function and underlying regulatory mechanism of PsnICE1 from Xiaohei poplar (Populus simonii x Populus nigra). PsnICE1 was significantly induced in response to cold stress in the roots, stems and leaves. PsnICE1 proteins were found to localize to the nucleus and exert transactivation activity via their N-terminal transactivation domain. Compared with non-transgenic poplar, transgenic poplar overexpressing PsnICE1 showed substantially enhanced tolerance to cold stress, with higher survival rates and antioxidant enzyme activity levels and reduced reactive oxygen species (ROS) accumulation. In contrast, plants with RNA inhibition-mediated silencing of PsnICE1 showed the opposite phenotype. PsnICE1 can bind to H-box element and abscisic acid-responsive element (ABRE), and more importantly, it mainly binds to IBS1 (a newly discovered cis-acting element) and E-box elements to regulate stress-related genes involved in ROS scavenging. Overall, these results indicated that PsnICE1 functions as a positive regulator of cold tolerance and serves as a potential candidate gene for plant cold tolerance improvement via molecular breeding.