文献类型：期刊文献

英文题名：Identification of major walnut cultivars grown in China based on nut phenotypes and SSR markers

作者：Chen, LingNa[1,2] Ma, QingGuo[2] Chen, YongKun[1] Wang, BaoQing[3] Pei, Dong[2]

第一作者：Chen, LingNa

通信作者：Pei, D[1]|[a0005c81e28dc32effca6]裴东;

机构：[1]Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing 100083, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Xinjiang Acad Forestry, Urumqi 830000, Xinjiang, Peoples R China

年份：2014

卷号：168

起止页码：240-248

外文期刊名：SCIENTIA HORTICULTURAE

收录：;Scopus(收录号：2-s2.0-84896832965);WOS:【SCI-EXPANDED(收录号:WOS:000335108600033)】；

基金：Financial support for this work was provided by the National Natural Science Foundation of China (Grant No. 31171933).

语种：英文

外文关键词：Walnut; Nut phenotype; Simple sequence repeats (SSR); Fingerprinting; Cultivar identification

摘要：Walnut (Juglans regia L) is an economically important tree widely cultivated in temperate regions for its nuts and timber. In this study, molecular identification using 12 simple sequence repeat (SSR) markers and 22 nut-shape characters were conducted on 35 cultivars of walnut grown in China. All 35 cultivars were classified into 11 broad categories on the basis of the nut phenotype. The shape characteristic of longitudinal sections to the suture presented a broad elliptic or elliptic shape was the largest category and included 18 cultivars. All accessions in each category were differentiated by no more than two SSR primer pairs. In addition to nut phenotype, 35 cultivars could be identified using four primer pairs, and every cultivar presented unique fingerprints. In particular, cultivars with the same parents were easily differentiated, such as 'Liaoning 1' and 'Liaoning 3' based on their different nut shape. Regardless of nut phenotype characters, just four loci (WJR031, WJR069, WJR265, and WJR281) also allowed the unambiguous separation of the 35 cultivars studied. This study provides a useful protocol for the characterization, identification, and authentication of any walnut cultivar and also shows the power of SSR markers in cultivar identification and intellectual property protection. Furthermore, the classification of nut shape is important for nut batch processing and for regional cultivar selection, which could be useful for the walnut industry. (C) 2014 Elsevier B.V. All rights reserved.