文献类型：期刊文献

英文题名：SaHsfA4c From Sedum alfredii Hance Enhances Cadmium Tolerance by Regulating ROS-Scavenger Activities and Heat Shock Proteins Expression

作者：Chen, Shuangshuang[1,2,3] Yu, Miao[1,2] Li, He[1,2] Wang, Ying[1,2] Lu, Zhuchou[1,2] Zhang, Yunxing[1,2] Liu, Mingying[1,2] Qiao, Guirong[1,2] Wu, Longhua[4] Han, Xiaojiao[1,2] Zhuo, Renying[1,2]

第一作者：Chen, Shuangshuang

通信作者：Han, XJ[1];Zhuo, RY[1];Han, XJ[2];Zhuo, RY[2]|[a00058eb5603bedf0c2e1]卓仁英;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Breeding Zhejiang Prov, Hangzhou, Peoples R China;[3]Jiangsu Acad Agr Sci, Inst Leisure Agr, Nanjing, Peoples R China;[4]Chinese Acad Sci, Inst Soil Sci, Natl Engn Lab Soil Pollut Control & Remediat Tech, Nanjing, Peoples R China

年份：2020

卷号：11

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000524733600001)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 31872168); the National Nonprofit Institute Research Grant of Chinese Academy of Forestry (CAFYBB2017ZY007 and CAFYBB2016SY008); and the National Key R&D Program of China (No. 2016YFD0800801).

语种：英文

外文关键词：Sedum alfredii Hance; heat shock transcription factor; cadmium stress; overexpression; ROS-scavenging enzyme

摘要：The heat shock transcription factor (Hsf) family, an important member in plant stress response, affects cadmium (Cd) tolerance in plants. In this study, we identified and functionally characterized a transcript of the Hsf A4 subgroup from Sedum alfredii. Designated as SaHsfA4c, the open reading frame was 1,302 bp long and encoded a putative protein of 433 amino acids containing a complete DNA-binding domain (DBD). Heterologous expression of SaHsfA4c in yeast enhanced Cd stress tolerance and accumulation, whereas expression of the alternatively spliced transcript InSaHsfA4c which contained an intron and harbored an incomplete DBD, resulted in relatively poor Cd stress tolerance and low Cd accumulation in transgenic yeast. The function of SaHsfA4c under Cd stress was characterized in transgenic Arabidopsis and non-hyperaccumulation ecotype S. alfredii. SaHsfA4c was able to rescue the Cd sensitivity of the Arabidopsis athsfa4c mutant. SaHsfA4c reduced reactive oxygen species (ROS) accumulation and increased the expression of ROS-scavenging enzyme genes and Hsps in transgenic lines. The present results suggest that SaHsfA4c increases plant resistance to stress by up-regulating the activities of ROS-scavenging enzyme and the expression of Hsps.