文献类型：期刊文献

中文题名：日喀则市贡觉林卡古树名木腐烂病的调查和诊断

英文题名：Investigation and Diagnose of the Ancient and Famous Tree Canker Diseases in Gongjuelinka Garden,Rikaze City

作者：姜宁[1] 万寿成[2] 薛寒[1] 李永[1]

第一作者：姜宁

机构：[1]中国林业科学研究院森林生态环境与自然保护研究所,生物多样性保护国家林业和草原局重点实验室,北京100091;[2]西藏日喀则市林业和草原局,日喀则857000

年份：2024

卷号：4

期号：4

起止页码：34-41

中文期刊名：陆地生态系统与保护学报

外文期刊名：Terrestrial Ecosystem and Conservation

基金：中国林业科学研究院基本科研业务费专项资助(CAFYBB2023PA002)。

语种：中文

中文关键词：壳囊孢;间座壳目;病害;系统学;分类

外文关键词：Cytospora;Diaporthales;disease;phylogeny;taxonomy

分类号：S718.8

摘要：【目的】掌握日喀则市贡觉林卡古树名木腐烂病的发生情况并明确病原菌的种类。【方法】于2022年夏季在贡觉林卡对杨柳树腐烂病进行调查采样,通过组织分离法获得病原菌菌株,并结合形态学和基于rDNA内转录间隔区(ITS)、肌动蛋白(act)、RNA合成酶第二亚基基因(rpb2)、转录延长因子基因(tef1)和微管蛋白(tub2)这5个基因片段的分子系统学研究,对分离自病斑的菌株进行准确鉴定。【结果】贡觉林卡内的藏川杨(Populus szechuanica var.tibetica)、白柳(Salix alba)、乌柳(S.cheilophila)和皂柳(S.wallichiana)枝干腐烂病发生普遍,发病率近60%。藏川杨腐烂病菌为杨生壳囊孢(Cytospora populicola);白柳腐烂病菌为臭椿生壳囊孢(C.ailanthicola)和杨小壳囊孢(C.populina);乌柳和皂柳腐烂病菌为金黄壳囊孢(C.chrysosperma)。【结论】明确了日喀则市古杨柳树腐烂病的病原菌,为该类病害的科学防控打下了基础。
【Objective】The aim of this study was to clarify the canker disease affecting ancient and famous trees in Gongjuelinka Garden,Rikaze City,and to identify the causative pathogens.【Methods】Investigations were carried out in Gongjuelinka Garden during the summer of 2022.Fungal isolates were obtained from diseased branches using tissue isolation methods.The isolates were identified through a combination of phylogenetic analysis based on ITS,act,rpb2,tef1 and tub2 sequence loci and morphological characterization.【Results】The canker disease incidence in Populus szechuanica var.tibetica,Salix alba,S.cheilophila and S.wallichiana was approximately 60%in Gongjuelinka Garden.Isolates from P.szechuanica var.tibetica were identified as Cytospora populicola;isolates from S.alba represented two species,C.ailanthicola and C.populina;and isolates from S.cheilophila and S.wallichiana were identified as C.chrysosperma.【Conclusion】This study identified the pathogens responsible for the canker disease affecting ancient and famous tree species in Rikaze City,which provides a foundation for disease management.