文献类型：期刊文献

中文题名：木质素代谢途径相关酚酸和类黄酮的HPLC测定方法的研究

英文题名：HPLC Measure of Phenolic acid and Flavonoid Relate to the Lignin Biosynthesis

作者：饶国栋[1] 张建国[1]

第一作者：饶国栋

机构：[1]中国林业科学研究院林业研究所/国家林业局林木培育重点实验室

年份：2013

卷号：29

期号：22

起止页码：46-50

中文期刊名：中国农学通报

外文期刊名：Chinese Agricultural Science Bulletin

收录：CSTPCD;;CSCD:【CSCD_E2013_2014】；

基金：北京市教育委员会科学研究与研究生培养共建项目"学科建设-森林培育"(20120618)

语种：中文

中文关键词：高效液相色谱;酚酸;类黄酮

外文关键词：HPLC; phenolic acid; flavonoid

分类号：S718.3

摘要：为了研究木质素代谢途径中相关酚酸和类黄酮的测定方法。采用有机试剂萃取和超声破碎细胞的方法对烟草总酚酸和类黄酮进行提取,并通过高效液相色谱(HPLC)对烟草中6种植物酚酸和5种植物类黄酮进行了定量分析。结果显示:有机萃取结合超声破碎细胞的方法能够将酚酸和类黄酮从植物中有效分离;高效液相色谱(HPLC)能够将6种植物酚酸和5种植物类黄酮一次分离;HPLC测定香豆酸、阿魏酸、芥子酸、对甲氧基苯甲酸和肉桂酸的保留时间分别为18.11、20.03、20.51、20.76、22.86、26.23min;HPLC测定杨梅素、槲皮素、根皮素、柑橘素、山奈酚的保留时间分别为9.06、12.01、14.96、15.23、16.12min。通过研究表明,HPLC能够很好的对植物酚酸和类黄酮进行检测,这为研究木质素代谢途径相关酚酸和类黄酮奠定了基础。
The aim was to establish a research method to measure the phenolic and flavonoid which relate to plant lignin biosynthesis. Organic reagent and ultrasonication were used for plant phenolic and flavonoid extract, HPLC was used for the measurement of six plant phenolic and five flavonoids. Tobacco was used as sample to test the HPLC measure method. The result showed that HPLC method for the phenolic and flavonoid relate to plant lignin biosynthesis was efficient which could isolate 6 different phenolics and 5 different flavonoids in one test; organic reagent and ultrasonication were useful to extract plant phenolic and flavonoid; the retention time of p-coumaric, ferulic, caffeic, cinnamic, sinapic and 4-methoxybenzoic acid were 18.11, 20.03, 20.51, 20.76, 22.86 and 26.23 min; the retention time of myricetin, quercetin, phloretin, naringenin and kaempferol were 9.06, 12.01, 14.96, 15.23, 16.12 min. The method made foundation for the research of plant lignin biosynthesis.