文献类型：期刊文献

英文题名：Arabidopsis AtPRP17 functions in embryo development by regulating embryonic patterning

作者：Shao, Dong Jie[1] Wei, Yi Ming[1] Yu, Zhong Qing[1] Dai, Xinren[2] Gao, Xin-Qi[1]

第一作者：Shao, Dong Jie

通信作者：Gao, XQ[1]

机构：[1]Shandong Agr Univ, Coll Life Sci, Natl Key Lab Crop Biol, Tai An 271018, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2021

卷号：254

期号：3

外文期刊名：PLANTA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000687685300001)】；

基金：This work was supported by the National Natural Science Foundation of China (31970190) and Youth Program of National Natural Science Foundation of China (31800267).

语种：英文

外文关键词：Arabidopsis; Seed; Embryonic patterning; Splicing factor

摘要：Main conclusion Arabidopsis AtPRP17, a homolog of yeast splicing factor gene PRP17, is expressed in siliques and embryos and functions in embryo development via regulating embryonic patterning. Yeast splicing factor PRP17/CDC40 is essential for cell growth through involvement in cell cycle regulation. Arabidopsis genome encodes a homolog of PRP17, AtPRP17; however, its function in Arabidopsis development is unknown. This study showed that AtPRP17 was highly expressed in siliques and embryos, and the protein was localized in the nucleus. The loss-of-function mutation of AtPRP17 led to shrunken seeds in Arabidopsis mature siliques. Further analysis revealed that the defective mature seeds of the mutant resulted from abnormal embryos with shriveled cotyledons, unequal cotyledons, swollen and shortened hypocotyls, or shortened radicles. During embryogenesis, mutant embryos showed delayed development and defective patterning of the apical and base domains, such as inhibited cotyledons and disorganized quiescent center cells and columella. Our results suggested that AtPRP17 functions in Arabidopsis embryo development via regulating embryonic patterning.