文献类型：期刊文献

英文题名：Plant commensal type VII secretion system causes iron leakage from roots to promote colonization

作者：Liu, Yunpeng[1] Shu, Xia[1,2,3] Chen, Lin[4] Zhang, Huihui[2] Feng, Haichao[2] Sun, Xiting[1] Xiong, Qin[1] Li, Guangqi[1] Xun, Weibing[2] Xu, Zhihui[2] Zhang, Nan[2] Pieterse, Corne M. J.[5] Shen, Qirong[2] Zhang, Ruifu[1,2]

第一作者：Liu, Yunpeng

通信作者：Liu, YP[1];Zhang, RF[1];Zhang, RF[2]

机构：[1]Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, State Key Lab Efficient Utilizat Arid & Semiarid A, Beijing, Peoples R China;[2]Nanjing Agr Univ, Natl Engn Res Ctr Organ Based Fertilizers, Jiangsu Collaborat Innovat Ctr Solid Organ Waste R, Jiangsu Prov Key Lab Organ Solid Waste Utilizat, Nanjing, Peoples R China;[3]Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R China;[4]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing, Peoples R China;[5]Univ Utrecht, Dept Biol, Plant Microbe Interact, Utrecht, Netherlands

年份：2023

外文期刊名：NATURE MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-85160422331);WOS:【SCI-EXPANDED(收录号:WOS:000996246800001)】；

基金：This work was funded by the National Natural Science Foundation of China (32070104, Y.L.), the National Natural Science Foundation of China (32172661, R.Z.), the National Key Research and Development Programme (2022YFF1001804, R.Z.), the National Key Research and Development Program (2021YFF1000400, Y.L. and R.Z.), the Central Public-interest Scientific Institution Basal Research Fund (No. Y2022QC15, Y.L.), and the Agricultural Science and Technology Innovation Program (CAAS-ZDRW202308, Y.L.). We thank H. Wei and J. Li of the Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, for providing the strains and plasmid used in the Agrobacterium-mediated transient expression experiment; and X. Shen of the College of Life Sciences, Northwest A&F University, for help with editing the manuscript.

语种：英文

摘要：Competition for iron is an important factor for microbial niche establishment in the rhizosphere. Pathogenic and beneficial symbiotic bacteria use various secretion systems to interact with their hosts and acquire limited resources from the environment. Bacillus spp. are important plant commensals that encode a type VII secretion system (T7SS). However, the function of this secretion system in rhizobacteria-plant interactions is unclear. Here we use the beneficial rhizobacterium Bacillus velezensis SQR9 to show that the T7SS and the major secreted protein YukE are critical for root colonization. In planta experiments and liposome-based experiments demonstrate that secreted YukE inserts into the plant plasma membrane and causes root iron leakage in the early stage of inoculation. The increased availability of iron promotes root colonization by SQR9. Overall, our work reveals a previously undescribed role of the T7SS in a beneficial rhizobacterium to promote colonization and thus plant-microbe interactions. The plant commensal Bacillus velezensis SQR9 uses its type VII secretion system to deliver effector protein YukE to plant roots, which causes iron leakage and promotes its colonization on roots.