文献类型：期刊文献

英文题名：Growth and Transcriptomics Analysis of Michelia macclurei Dandy Plantlets with Different LED Quality Treatments

作者：Chen, Zhaoli[1] Liu, Ying[1] Zeng, Bingshan[1] Jiang, Qingbin[1] Wang, Shengkun[1] Li, Xiangyang[1]

通信作者：Liu, Y[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Peoples R China

年份：2023

卷号：92

期号：10

起止页码：2891-2906

外文期刊名：PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001108662400003)】；

基金：This research was funded and supported by Fundamental Research Funds of the Chinese Academy of Forestry, No. CAFYBB2017MB10.

语种：英文

外文关键词：Michelia macclurei; plantlets; growth characteristics; transcriptome

摘要：Michelia macclurei Dandy is a significant tree species that has extensive cultivation for forestry and horticulture purposes in southern China, owing to its economic and practical importance. Light quality influences plantlet growth and development during tissue culture. However, the growth characteristic and molecular regulation of M. macclurei under different light quality conditions are not well understood yet. In this study, we investigated the morphological, chlorophyll content, and transcriptomic responses of M. macclurei plantlets under different light-emitting diode (LED) qualities, including white, blue, and red light. The results showed that blue light significantly increased plant height (21.29%) and leaf number (18.65%), while red light decreased plant height and leaf number by 7.53% and 16.49%, respectively. In addition, the plantlets' chlorophyll content and etiolation rate were significantly reduced by blue and red light quality compared to white light. Compared to white light, blue light had a negative effect, leading to decreased rooting rate (64.28%), root number (72.72%), and root length (75.86%). Conversely, red light had a positive effect, resulting in increased rooting rate (24.99%), root number (109.58%), and root length (72.72%). Transcriptome analysis identified 54 differentially expressed genes (DEGs) in three groups that consisted of blue light vs. white light (BL-vs-WL), red light vs. white light (RL-vs-WL), and red light vs. blue light (RL-vs-BL). Specifically, 21, 7, and 41 DEGs were identified in the three groups, respectively. The DEGs found in the RL-vs-WL and BL-vs-WL groups were involved in plant hormone signaling, nitro-gen metabolism, and phenylpropanoid biosynthesis pathways, which suggests that M. macclurei plantlets adapt to the changes of light quality via modulating gene expression. Overall, our study provides valuable insights for understanding the molecular and morphological responses of M. macclurei plantlets under different light qualities.