文献类型：期刊文献

英文题名：Multilocus sequence analysis of the Rhizobia from five woody legumes in southern China

作者：Lu, Junkun[1] Kang, Lihua[] He, Xinhua[2,3] Xu, Daping[1]

第一作者：陆俊锟

通信作者：Xu, DP[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China;[2]Univ Western Australia, State Ctr Excellence Ecohydrol, Crawley, WA 6009, Australia;[3]Univ Western Australia, Sch Plant Biol, Crawley, WA 6009, Australia

年份：2011

卷号：5

期号：30

起止页码：5343-5353

外文期刊名：AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000298924100004)】；

基金：This study was financially supported by projects from the Forestry Social Benefit Science and Technology for Special Fund (201004075), the Guangdong Forestry Science and Technology for Creativity and Special Fund (2009KJCX002-01), and the National Natural Science Foundation of China (31170582). We thank Professor Bernard Dell of Murdoch University, Australia, and Doctor Chen Wenfeng of China Agricultural University for their valuable comments on this manuscript.

语种：英文

外文关键词：Multilocus sequence analysis; 16S rRNA; rhizobia and woody legume

摘要：A total of 11 rhizobial strains from five woody legumes (Acacia confusa, Dalbergia odorifera, Dalbergia fusca, Erythrophleum fordii and Pterocarpus macarocarpus) in Southern China were identified, using 16S rRNA gene sequence analysis and multilocus sequence analysis (MLSA) of three housekeeping genes (recA, glnII and dnaK). Among the 11 isolated rhizobia, seven were classified as Agrobacterium tumefaciens, Bradyrhizobium elkanii, Ensifer adhaerens and Rhizobium multihospitium and four were within the genus Rhizobium that might be novel species when considering their clustering independently in topology tree and relatively low recA gene sequence similarities(<94%) to the recognized species. In each gene phylogeny, the 16S rRNA gene was found to be incongruent with each house-keeping gene, indicated that this gene should not be used as a single marker in rhizobial taxonomy. In addition, more robust topology trees were produced through the analysis of a concatenation of three housekeeping genes than through each housekeeping gene and 16S rRNA gene trees, with high bootstrap support (>= 55%). Overall, our results support that the MLSA has higher discrimination potential in rhizobial taxonomy than the 16S rRNA gene and is suitable to estimate phylogenetic relationships among rhizobia species.