文献类型：期刊文献

英文题名：Proteome-Level Analysis of Metabolism- and Stress-Related Proteins during Seed Dormancy and Germination in Gnetum parvifolium

作者：Chang, Ermei[1] Deng, Nan[2] Zhang, Jin[1] Liu, Jianfeng[1] Chen, Lanzhen[3,4] Zhao, Xiulian[1] Abbas, M.[1,5] Jiang, Zeping[1] Shi, Shengqing[1]

第一作者：Chang, Ermei

通信作者：Jiang, Zeping

机构：[1] State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, No. 1 Dongxiaofu, Haidian, Beijing, 100091, China; [2] Institute of Ecology, Hunan Academy of Forestry, Changsha, Hunan, 410004, China; [3] Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, 100093, China; [4] Risk Assessment Laboratory for Bee Products, Quality and Safety of Ministry of Agriculture, Beijing, 100093, China; [5] National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China

年份：2018

卷号：66

期号：11

起止页码：3019-3029

外文期刊名：Journal of Agricultural and Food Chemistry

收录：EI(收录号：20181304936903);Scopus(收录号：2-s2.0-85044221499)

语种：英文

外文关键词：Superoxide dismutase - Germination - Metabolism - Signal transduction - Antioxidants - Cluster analysis - Gene Ontology - Oils and fats - Plants (botany) - Starch

摘要：Gnetum parvifolium is a rich source of materials for traditional medicines, food, and oil, but little is known about the mechanism underlying its seed dormancy and germination. In this study, we analyzed the proteome-level changes in its seeds during germination using isobaric tags for relative and absolute quantitation. In total, 1,040 differentially expressed proteins were identified, and cluster analysis revealed the distinct time points during which signal transduction and oxidation-reduction activity changed. Gene Ontology analysis showed that "carbohydrate metabolic process" and "response to oxidative stress" were the main enriched terms. Proteins associated with starch degradation and antioxidant enzymes were important for dormancy-release, while proteins associated with energy metabolism and protein synthesis were up-regulated during germination. Moreover, protein-interaction networks were mainly associated with heat-shock proteins. Furthermore, in accord with changes in the energy metabolism- and antioxidant-related proteins, indole-3-acetic acid, Peroxidase, and soluble sugar content increased, and the starch content decreased in almost all six stages of dormancy and germination analyzed (S1-S6). The activity of superoxide dismutase, abscisic acid, and malondialdehyde content increased in the dormancy stages (S1-S3) and then decreased in the germination stages (S4-S6). Our results provide new insights into G. parvifolium seed dormancy and germination at the proteome and physiological levels, with implications for improving seed propagation. ? 2018 American Chemical Society.