文献类型：期刊文献

英文题名：Full-Length Transcriptome Sequencing and Different Chemotype Expression Profile Analysis of Genes Related to Monoterpenoid Biosynthesis in Cinnamomum porrectum

作者：Qiu, Fengying[1,2] Wang, Xindong[2] Zheng, Yongjie[2] Wang, Hongming[3] Liu, Xinliang[2] Su, Xiaohua[1,4]

第一作者：Qiu, Fengying

通信作者：Su, XH[1];Su, XH[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Forestry, Beijing 100091, Peoples R China;[2]Jiangxi Acad Forestry, Natl Forestry & Grassland Adm, Camphor Engn Technol Res Ctr, Nanchang 30032, Jiangxi, Peoples R China;[3]Tianshui Normal Univ, Coll Bioengn & Biotechnol, Tianshui 741000, Peoples R China;[4]Natl Forestry & Grassland Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China

年份：2019

卷号：20

期号：24

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000506840100124)】；

基金：This work was supported by the Technology Program of Jiangxi Academy of Forestry (2018512701); The National Key R&D Program (2016YFD0600605); Key R&D Projects of Jiangxi Province (20161ACF60021).

语种：英文

外文关键词：Cinnamomum porrectum; terpenoids; terpenoid biosynthesis; full-length transcriptome; different chemotypes

摘要：Leaves of C. porrectum are rich in essential oils containing monoterpenes, sesquiterpenes and aromatic compounds, but the molecular mechanism of terpenoid biosynthesis in C. porrectum is still unclear. In this paper, the differences in the contents and compositions of terpenoids among three chemotypes were analyzed using gas chromatography mass spectrometry (GC/MS). Furthermore, the differential expression of gene transcripts in the leaf tissues of the three C. porrectum chemotypes were analyzed through a comparison of full-length transcriptomes and expression profiles. The essential oil of the three C. porrectum chemotypes leaves was mainly composed of monoterpenes. In the full-length transcriptome of C. porrectum, 104,062 transcripts with 306,337,921 total bp, an average length of 2944 bp, and an N50 length of 5449 bp, were obtained and 94025 transcripts were annotated. In the eucalyptol and linalool chemotype, the camphor and eucalyptol chemotype, and the camphor and linalool chemotype comparison groups, 21, 22 and 18 terpene synthase (TPS) unigenes were identified respectively. Three monoterpene synthase genes, CpTPS3, CpTPS5 and CpTPS9, were upregulated in the eucalyptol chemotype compared to the linalool chemotype and camphor chemotype. CpTPS1 was upregulated in the camphor chemotype compared to the linalool chemotype and the eucalyptol chemotype. CpTPS4 was upregulated in the linalool chemotype compared to the camphor chemotype and the eucalyptol chemotype. Different unigenes had different expression levels among the three chemotypes, but the unigene expression levels of the 2-C-methyl-D-erythritol 4phosphate (MEP) pathway were generally higher than those of the mevalonate acid (MVA) pathway. Quantitative reverse transcription PCR(qRT-PCR) further validated these expression levels. The present study provides new clues for the functional exploration of the terpenoid synthesis mechanism and key genes in different chemotypes of C. porrectum.