文献类型：期刊文献

英文题名：Effects of feeding Clostera anachoreta on hydrogen peroxide accumulation and activities of peroxidase, catalase, and ascorbate peroxidase in Populus simonii x P. pyramidalis 'Opera 8277' leaves

作者：Hu, Zeng-Hui[1] Shen, Ying-Bai[1] Shen, Fan-Yi[2] Su, Xiao-Hua[3]

第一作者：Hu, Zeng-Hui

通信作者：Shen, YB[1]

机构：[1]Beijing Forestry Univ, Coll Biol Sci & Technol, Minist Educ, Key Lab Genet & Breeding Forest Trees & Ornamenta, Beijing 100083, Peoples R China;[2]Beijing Forestry Univ, Coll Sci, Beijing 100083, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China

年份：2009

卷号：31

期号：5

起止页码：995-1002

外文期刊名：ACTA PHYSIOLOGIAE PLANTARUM

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000269878700013)】；

基金：This work was collectively supported by the Program for Changjiang Scholars and Innovative Research Team in Universities of China (PCSIRT0607), by the Key Science Program of the State Forestry Administration of China (2006-59) and National Key Project of Scientific and Technical Supporting Programs Funded by the Ministry of Science & Technology of China (2006BAD01A15; 2006BAD24B04).

语种：英文

外文关键词：Activities of H2O2-scavenging enzymes; Clostera anachoreta; Hydrogen peroxide; Populus simonii x P. pyramidalis 'Opera 8277'

摘要：In response to Clostera anachoreta larvae attack, poplar (Populus simonii x P. pyramidalis 'Opera 8277') leaves produced a high level of hydrogen peroxide (H2O2). Histochemical localization revealed that H2O2 was mainly localized in herbivore-wounded zones and might spread through the veins. The activities of three H2O2-scavenging enzymes, i.e., peroxidase (POD), ascorbate peroxidase (APX), and catalase (CAT), were also enhanced in herbivore-wounded leaves, and exhibited an opposite pattern to the accumulation of H2O2. It was found that diphenylene iodonium chloride (DPI, a special inhibitor of NADPH oxidase) treatment significantly inhibited the accumulation of H2O2 induced by herbivory damage. Moreover, DPI treatment led to an obvious decrease in the activities of POD, APX, and CAT. The results indicated that NADPH oxidase contributed to the accumulation of H2O2 and the increase in activities of H2O2-scavenging enzymes in poplar leaves induced by herbivory damage. The balance between H2O2-production pathway and H2O2-scavenging enzymes led to the tolerable level of H2O2 acting in P. simonii x P. pyramidalis 'Opera 8277' cuttings in response to herbivory damage.