文献类型：期刊文献

中文题名：基于分子标记技术的马尾松和火炬松杂交子代鉴定与抗性评价

英文题名：Identification and Resistance Evaluation of Hybrid Offspring of Pinus massoniana and Pinus taeda Based on molecular Marker technology

作者：李俊[1,2] 刘青华[1] 高凯[1] 张振[1] 丰忠平[3] 周志春[1] 金国庆[1]

第一作者：李俊

机构：[1]中国林业科学研究院亚热带林业研究所林木遗传育种全国重点实验室全省林木育种重点实验室国家林业草原马尾松工程技术研究中心,浙江杭州311400;[2]四川农业大学,四川成都611130;[3]淳安县林业总场有限公司姥山分场,浙江杭州311700

年份：2025

卷号：38

期号：1

起止页码：181-189

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：;北大核心:【北大核心2023】；

基金：科技创新2030-重大项目(2022ZD04016);“十四五”国家重点研发计划项目(2022YFD2200202);浙江省农业新品种选育科技重大专项子课题“抗松材线虫病马尾松新品种选育”(2021C02070-5-2)。

语种：中文

中文关键词：马尾松×火炬松;分子标记;杂种鉴定;抗性育种

外文关键词：P.massoniana×P.taeda;molecular markers;hybrid identification;resistance breeding

分类号：S763.1

摘要：[目的]早期快速精准鉴定马尾松和火炬松杂交子代,选育高抗马尾松×火炬松家系,以期更好地防控松材线虫病。[方法]本研究以马尾松为母本,火炬松为父本进行杂交,比对火炬松和油松基因组序列设计特异性引物,筛选马尾松与火炬松种间特异性引物,对杂交后代进行扩增条带分析,建立马尾松×火炬松快速鉴定体系,通过接种松材线虫评价筛选高抗家系。[结果]从150对引物中筛选出12对引物组合用于鉴定,特异性强,稳定性好,且扩增率高。F1代29个家系共88个样本,其中81株鉴定为杂交植株,杂种率为92.05%;F2代21个家系共688个样本,其中279株为杂交植株,杂种率为40.55%;对马尾松×火炬松F2代13个家系进行抗性评价,获得5个高抗家系。[结论]12对引物组合可作为马尾松和火炬松杂交子代真实性鉴定,筛选获得5个高抗家系,为松材线虫病防控和抗性机理研究奠定基础。
[Purpose]The early,rapid and accurate identification of hybrid offspring between Pinus massoniana and P.taeda,and the breeding of high resistant P.massoniana×P.taeda family are of great significance for the prevention and control of pine wilt disease.[Method]This study used P.massoniana as the female parent and P.taeda as the male parent for hybridization.Specific primers were designed by comparing the genome sequences of P.taeda and P.tabulaeformis.Specific primers between P.massoniana and P.taeda were screened,and amplification band analysis was performed on the hybrid offspring to establish a rapid identification system for P.massoniana×P.taeda.Families with high resistance were evaluated and screened by inoculating with pine wood nematodes.[Result]12 primer combinations were selected from 150 pairs for identification,with strong specificity,good stability,and high amplification rate.A total of 88 samples were collected from 29 families in the F1 generation,of which 81 were identified as hybrid plants with a hybrid rate of 92.05%;There were a total of 688 samples from 21 families in the F2 generation,of which 279 were hybrid plants with a hybrid rate of 40.55%;5 highly resistant families were identified from the resistance evaluation of 13 families in the F2 generation of P.massoniana×P.taeda,resulting in.[Conclusion]The 12 selected primer combinations effectively authenticate hybrid offspring of P.massoniana and P.taeda.Furthermore,5 highly resistant families were identified,providing a foundation for pine wilt disease prevention,control,and further research into resistance mechanisms.