文献类型：期刊文献

英文题名：Functional Diversification of the Dihydroflavonol 4-Reductase from Camellia nitidissima Chi. in the Control of Polyphenol Biosynthesis

作者：Jiang, Lina[1,2] Fan, Zhengqi[1,2] Tong, Ran[1] Zhou, Xingwen[3] Li, Jiyuan[1] Yin, Hengfu[1,2]

第一作者：Jiang, Lina

通信作者：Li, JY[1];Yin, HF[1];Yin, HF[2]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, State Key Lab Tree Genet & Breeding, Hangzhou 311400, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Forest Genet & Breeding, Hangzhou 311400, Peoples R China;[3]Yulin Normal Univ, Coll Biol & Pharm, Yulin 537000, Peoples R China

年份：2020

卷号：11

期号：11

起止页码：1-12

外文期刊名：GENES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000594026700001)】；

基金：This work was supported by the National key projects for international scientific and technological innovation cooperation among governments [grant numbers 2016 YFE0126100]; and the special funds for basic scientific research expenses of public welfare research institutes of the Chinese academy of forestry [grant number CAFYBB2017 ZF001].

语种：英文

外文关键词：floral pigmentation; dihydroflavonol 4-reduetase; Camellia nitidissima Chi; polyphenols; anthocyanins

摘要：Plant secondary metabolism is complex in its diverse chemical composition and dynamic regulation of biosynthesis. How the functional diversification of enzymes contributes to the diversity is largely unknown. In the flavonoids pathway, dihydroflavonol 4-reductase (DFR) is a key enzyme mediating dihydroflavanol into anthocyanins biosynthesis. Here, the DFR homolog was identified from Camellia nitidissima Chi. (CnDFR) which is a unique species of the genus Camellia with golden yellow petals. Sequence analysis showed that CnDFR possessed not only conserved catalytic domains, but also some amino acids peculiar to Camellia species. Gene expression analysis revealed that CnDFR was expressed in all tissues and the expression of CnDFR was positively correlated with polyphenols but negatively with yellow coloration. The subcellular localization of CnDFR by the tobacco infiltration assay showed a likely dual localization in the nucleus and cell membrane. Furthermore, overexpression transgenic lines were generated in tobacco to understand the molecular function of CnDFR. The analyses of metabolites suggested that ectopic expression of CnDFR enhanced the biosynthesis of polyphenols, while no accumulation of anthocyanins was detected. These results indicate a functional diversification of the reductase activities in Camellia plants and provide molecular insights into the regulation of floral color.