文献类型：期刊文献

中文题名：4个桉树无性系愈伤诱导和分化

英文题名：Induction and Differentiation of Four Eucalyptus Clones in vitro Culture

作者：裘珍飞[1] 曾炳山[1] 李湘阳[1] 刘英[1] 查志刚[2]

第一作者：裘珍飞

机构：[1]中国林业科学研究院热带林业研究所;[2]安徽黄山市森林病虫防治检疫站

年份：2012

卷号：25

期号：4

起止页码：531-534

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

基金："863"项目"白桦;桉树等分子育种与品种创制"(2011AA100202)研究内容

语种：中文

中文关键词：桉树;愈伤诱导;再生率

外文关键词：Eucalyptus ; callus induction ; regeneration rate

分类号：S723.1

摘要：桉树（Eucalyptus L＇Hérit.）是世界三大树种之一,随着生物技术的发展,桉树转基因研究正以前所未有的速度发展,而不同的种、无性系胚性愈伤诱导的难易和植株再生能力的强弱直接影响转基因工程实施的进程。尾巨桉（E.urophylla S.T.Blake×E.grandis W.Hill ex Maiden）无性系DH3229、
The in vitro p used as explants to study lantlet leaves and stems of 4 elite Eucalyptus clones commonly cultivated in production were the callus induction and differentiation under different callus induction time, in order to establish an effective callus regeneration system. The results showed ： The clones Eg5 and DH201-2 leaf callus inducted for 45 d were suitable for differentiation of adventitious buds, with the highest regeneration rate of 70.0% and 63.3% respectively. 20 d and 15 d were the best induction times for leaf callus inducting and stem callus inducting of clone GL9, with the regeneration rates of 66.7% and 63.3% respectively. The best regeneration rate of DH3229 was 16.7% from stem callus inducted for 30 d. There were significant differences in regeneration rate among the 4 clones. Clones EgS, DH201-2, and GL9 were easy to regenerate compared with DH3229. No obvious difference was found in rezeneration rate of leaf callus and stem callus from same clone.